A New Gnotobiotic Pig Model of P[6] Human Rotavirus Infection and Disease for Preclinical Evaluation of Rotavirus Vaccines

Simple item page
dc.contributor.authorNyblade, Charlotteen
dc.contributor.authorHensley, Caseyen
dc.contributor.authorParreƱo, Vivianaen
dc.contributor.authorZhou, Pengen
dc.contributor.authorFrazier, Maggieen
dc.contributor.authorFrazier, Annieen
dc.contributor.authorRamesh, Ashwinen
dc.contributor.authorLei, Shaohuaen
dc.contributor.authorDegiuseppe, Juan Ignacioen
dc.contributor.authorTan, Mingen
dc.contributor.authorYuan, Lijuanen
dc.date.accessioned2022-12-22T14:59:10Zen
dc.date.available2022-12-22T14:59:10Zen
dc.date.issued2022-12-15en
dc.date.updated2022-12-22T14:35:05Zen
dc.description.abstractHuman rotavirus (HRV) is a leading cause of gastroenteritis in children under 5 years of age. Licensed vaccines containing G1P[8] and G1-4P[8] strains are less efficacious against newly emerging P[6] strains, indicating an urgent need for better cross protective vaccines. Here, we report our development of a new gnotobiotic (Gn) pig model of P[6] HRV infection and disease as a tool for evaluating potential vaccine candidates. The Arg HRV (G4P[6]) strain was derived from a diarrheic human infant stool sample and determined to be free of other viruses by metagenomic sequencing. Neonatal Gn pigs were orally inoculated with the stool suspension containing 5.6 &times; 10<sup>5</sup> fluorescent focus units (FFU) of the virus. Small and large intestinal contents were collected at post inoculation day 2 or 3. The virus was passaged 6 times in neonatal Gn pigs to generate a large inoculum pool. Next, 33&ndash;34 day old Gn pigs were orally inoculated with 10<sup>&minus;2</sup>, 10<sup>3</sup>, 10<sup>4</sup>, and 10<sup>5</sup> FFU of Arg HRV to determine the optimal challenge dose. All pigs developed clinical signs of infection, regardless of the inoculum dose. The optimal challenge dose was determined to be 10<sup>5</sup> FFU. This new Gn pig model is ready to be used to assess the protective efficacy of candidate monovalent and multivalent vaccines against P[6] HRV.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationNyblade, C.; Hensley, C.; ParreƱo, V.; Zhou, P.; Frazier, M.; Frazier, A.; Ramesh, A.; Lei, S.; Degiuseppe, J.I.; Tan, M.; Yuan, L. A New Gnotobiotic Pig Model of P[6] Human Rotavirus Infection and Disease for Preclinical Evaluation of Rotavirus Vaccines. Viruses 2022, 14, 2803.en
dc.identifier.doihttps://doi.org/10.3390/v14122803en
dc.identifier.urihttp://hdl.handle.net/10919/112982en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjecthuman rotavirusen
dc.subjectP[6] genotypeen
dc.subjectgnotobiotic pig modelen
dc.subjectdiarrheaen
dc.subjectvaccine evaluationen
dc.titleA New Gnotobiotic Pig Model of P[6] Human Rotavirus Infection and Disease for Preclinical Evaluation of Rotavirus Vaccinesen
dc.title.serialVirusesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
viruses-14-02803.pdf
Size:
2.74 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
0 B
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Journal Articles, Multidisciplinary Digital Publishing Institute (MDPI)
Scholarly Works, Biomedical Sciences and Pathobiology