Altered phosphorylation, electrophysiology, and behavior on attenuation of PDE4B action in hippocampus

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dc.contributor.authorCampbell, Susan L.en
dc.contributor.authorvan Groen, Thomasen
dc.contributor.authorKadish, Ingaen
dc.contributor.authorSmoot, Lisa High Mitchellen
dc.contributor.authorBolger, Graeme B.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2019-01-22T15:40:43Zen
dc.date.available2019-01-22T15:40:43Zen
dc.date.issued2017en
dc.description.abstractBackground: PDE4 cyclic nucleotide phosphodiesterases regulate 3′, 5′ cAMP abundance in the CNS and thereby regulate PKA activity and phosphorylation of CREB, which has been implicated in learning and memory, depression and other functions. The PDE4 isoform PDE4B1 also interacts with the DISC1 protein, implicated in neural development and behavioral disorders. The cellular functions of PDE4B1 have been investigated extensively, but its function(s) in the intact organism remained unexplored. Results: To specifically disrupt PDE4B1, we developed mice that express a PDE4B1-D564A transgene in the hippocampus and forebrain. The transgenic mice showed enhanced phosphorylation of CREB and ERK1/2 in hippocampus. Hippocampal neurogenesis was increased in the transgenic mice. Hippocampal electrophysiological studies showed increased baseline synaptic transmission and enhanced LTP in male transgenic mice. Behaviorally, male transgenic mice showed increased activity in prolonged open field testing, but neither male nor female transgenic mice showed detectable anxiety-like behavior or antidepressant effects in the elevated plus-maze, tail-suspension or forced-swim tests. Neither sex showed any significant differences in associative fear conditioning or showed any demonstrable abnormalities in pre-pulse inhibition. Conclusions: These data support the use of an isoform-selective approach to the study of PDE4B1 function in the CNS and suggest a probable role of PDE4B1 in synaptic plasticity and behavior. They also provide additional rationale and a refined approach to the development of small-molecule PDE4B1-selective inhibitors, which have potential functions in disorders of cognition, memory, mood and affect.en
dc.description.sponsorshipThe McKnight Foundation (no grant number, for research support and also funding of the behavioral and electrophysiology cores), the Bolger Prostate Cancer Research Fund (no grant number), and the National Cancer Institute of the National Institutes of Health to the University of Alabama at Birmingham Comprehensive Cancer Center under award number P30 CA013148 (for generation of transgenic mice and DNA sequencing). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or the McKnight Foundation, neither of which played any role on the in study design; in the collection, analysis, and interpretation of data; in the writing of the manuscript; or in the decision to submit the manuscript for publication.en
dc.description.versionPublished versionen
dc.format.extent20 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationCampbell et al. BMC Neurosci (2017) 18:77 https://doi.org/10.1186/s12868-017-0396-6en
dc.identifier.doihttps://doi.org/10.1186/s12868-017-0396-6en
dc.identifier.issue1en
dc.identifier.urihttp://hdl.handle.net/10919/86825en
dc.identifier.volume18en
dc.language.isoenen
dc.publisherBMCen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLearningen
dc.subjectMemoryen
dc.subjectDepressionen
dc.subjectPKAen
dc.subjectCREBen
dc.subjectDISC1en
dc.subjectPDE4en
dc.subjectPDE4B1en
dc.titleAltered phosphorylation, electrophysiology, and behavior on attenuation of PDE4B action in hippocampusen
dc.title.serialBMC Neuroscienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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