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Arabidopsis UMAMIT24 and 25 are amino acid exporters involved in seed loading

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dc.contributor.authorBesnard, Julienen
dc.contributor.authorZhao, Chengsongen
dc.contributor.authorAvice, Jean-Christopheen
dc.contributor.authorVitha, Stanislaven
dc.contributor.authorHyodo, Ayumien
dc.contributor.authorPilot, Guillaumeen
dc.contributor.authorOkumoto, Sakikoen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2021-08-03T13:09:17Zen
dc.date.available2021-08-03T13:09:17Zen
dc.date.issued2018-10-12en
dc.date.updated2021-08-03T13:09:14Zen
dc.description.abstractPhloem-derived amino acids are the major source of nitrogen supplied to developing seeds. Amino acid transfer from the maternal to the filial tissue requires at least one cellular export step from the maternal tissue prior to the import into the symplasmically isolated embryo. Some members of UMAMIT (usually multiple acids move in an out transporter) family (UMAMIT11, 14, 18, 28, and 29) have previously been implicated in this process. Here we show that additional members of the UMAMIT family, UMAMIT24 and UMAMIT25, also function in amino acid transfer in developing seeds. Using a recently published yeast-based assay allowing detection of amino acid secretion, we showed that UMAMIT24 and UMAMIT25 promote export of a broad range of amino acids in yeast. In plants, UMAMIT24 and UMAMIT25 are expressed in distinct tissues within developing seeds; UMAMIT24 is mainly expressed in the chalazal seed coat and localized on the tonoplast, whereas the plasma membrane-localized UMAMIT25 is expressed in endosperm cells. Seed amino acid contents of umamit24 and umamit25 knockout lines were both decreased during embryogenesis compared with the wild type, but recovered in the mature seeds without any deleterious effect on yield. The results suggest that UMAMIT24 and 25 play different roles in amino acid translocation from the maternal to filial tissue; UMAMIT24 could have a role in temporary storage of amino acids in the chalaza, while UMAMIT25 would mediate amino acid export from the endosperm, the last step before amino acids are taken up by the developing embryo.en
dc.description.versionPublished versionen
dc.format.extentPages 5221-5232en
dc.format.extent12 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1093/jxb/ery302en
dc.identifier.eissn1460-2431en
dc.identifier.issn0022-0957en
dc.identifier.issue21en
dc.identifier.orcidPilot, Guillaume [0000-0001-7520-1059]en
dc.identifier.other5078731 (PII)en
dc.identifier.pmid30312461 (pubmed)en
dc.identifier.urihttp://hdl.handle.net/10919/104554en
dc.identifier.volume69en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000448163900019&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectPlant Sciencesen
dc.subjectAmino acidsen
dc.subjectArabidopsisen
dc.subjectmembrane exporten
dc.subjectNitrogenen
dc.subjectseeden
dc.subjecttransporteren
dc.subjectUMAMITen
dc.subjectEndoplasmic-Reticulumen
dc.subjectSaccharomyces-Cerevisiaeen
dc.subjectPhloem Transporten
dc.subjectBrassica-Napusen
dc.subjectExpressionen
dc.subjectThalianaen
dc.subjectProteinen
dc.subjectGeneen
dc.subjectEmbryosen
dc.subjectPlantsen
dc.subjectPlant Biology & Botanyen
dc.subject0604 Geneticsen
dc.subject0607 Plant Biologyen
dc.subject0703 Crop and Pasture Productionen
dc.subject.meshArabidopsisen
dc.subject.meshSeedsen
dc.subject.meshAmino Acidsen
dc.subject.meshGene Expression Profilingen
dc.titleArabidopsis UMAMIT24 and 25 are amino acid exporters involved in seed loadingen
dc.title.serialJournal of Experimental Botanyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2018-08-14en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen

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