Enhanced recovery of injured and noninjured cells of Bifidobacterium species from water and dairy products

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dc.contributor.authorArany, Catherine Beatriceen
dc.contributor.committeechairHackney, Cameron Rajen
dc.contributor.committeememberPierson, Merle D.en
dc.contributor.committeememberEigel, William N. IIIen
dc.contributor.departmentFood Science and Technologyen
dc.date.accessioned2014-03-14T21:51:29Zen
dc.date.adate2009-12-16en
dc.date.available2014-03-14T21:51:29Zen
dc.date.issued1992-09-03en
dc.date.rdate2009-12-16en
dc.date.sdate2009-12-16en
dc.description.abstract<u>Bifidobacterium</u> spp. are anaerobic, Gram-positive, nonmotile bacteria that are a major component of intestinal microbiota. They are potential indicators of human fecal pollution in shellfish harvesting waters. In addition, <u>Bifidobacterium</u> spp. are used as supplements in dairy products. These bacteria are easily injured and it is important to have methods that will recover all cells (injured and uninjured) present in water and food samples. The objectives of this research were to develop a repair detection (RD) procedure to be used with VPI's anaerobic roll tube apparatus for improving the recovery of Bifidobacterium and to compare this method with existing enumeration methods. <u>Bifidobacterium adolescentis</u> cells were injured by exposure to pond water. Cells were enumerated using a modification of Human Bifid Sorbitol Agar (MHBSA). MHBSA was modified by substituting phenyl red for bromocresol purple and adding methylene blue as an indicator of oxidation/reduction.en
dc.description.degreeMaster of Scienceen
dc.format.extentxi, 91 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-12162009-020131en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-12162009-020131/en
dc.identifier.urihttp://hdl.handle.net/10919/46232en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1992.A736.pdfen
dc.relation.isformatofOCLC# 27372449en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1992.A736en
dc.subject.lcshBifidobacteriumen
dc.titleEnhanced recovery of injured and noninjured cells of Bifidobacterium species from water and dairy productsen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineFood Science and Technologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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