Resistance exercise training and in vitro skeletal muscle oxidative capacity in older adults

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dc.contributor.authorFlack, Kyle D.en
dc.contributor.authorDavy, Brenda M.en
dc.contributor.authorDeBerardinis, Martinen
dc.contributor.authorBoutagy, Nabil E.en
dc.contributor.authorMcMillan, Ryan P.en
dc.contributor.authorHulver, Matthew W.en
dc.contributor.authorFrisard, Madlyn I.en
dc.contributor.authorAnderson, Angela S.en
dc.contributor.authorSavla, Jyoti S.en
dc.contributor.authorDavy, Kevin P.en
dc.contributor.departmentHuman Nutrition, Foods, and Exerciseen
dc.date.accessioned2016-11-17T20:55:21Zen
dc.date.available2016-11-17T20:55:21Zen
dc.date.issued2016-07en
dc.description.abstractWhether resistance exercise training (RET) improves skeletal muscle substrate oxidative capacity and reduces mitochondrial production of reactive oxygen species in older adults remains unclear. To address this, 19 older males (≥60 years) were randomized to a RET (n = 11) or to a waitlist control group (n = 8) that remained sedentary for 12 weeks. RET was comprised of three upper body and four lower body movements on resistance machines. One set of 8-12 repetitions to failure of each movement was performed on three nonconsecutive days/week. Improvements in chest press and leg press strength were assessed using a three-repetition maximum (3 RM). Body composition was assessed via dual energy X-ray absorptiometry. Muscle biopsies were obtained from the vastus lateralis muscle at baseline and at both 3 weeks and 12 weeks. Palmitate and pyruvate oxidation rates were measured from the (14)CO2 produced from [1-(14)C] palmitic acid and [U-(14)C] pyruvate, respectively, during incubation of muscle homogenates. PGC-1α, TFAM, and PPARδ levels were quantified using qRT-PCR Citrate synthase (CS) and β-HAD activities were determined spectrophotometrically. Mitochondrial production of reactive oxygen species (ROS) were assessed using the Amplex Red Hydrogen Peroxide/Peroxidase assay. There were no significant changes in body weight or body composition following the intervention. Chest press and leg press strength (3RM) increased ~34% (both P < 0.01) with RET There were no significant changes in pyruvate or fatty acid oxidation or in the expression of target genes with the intervention. There was a modest increase (P < 0.05) in βHAD activity with RET at 12 weeks but the change in CS enzyme activity was not significant. In addition, there were no significant changes in ROS production in either group following RET Taken together, the findings of this study suggest that 12 weeks of low volume RET does not increase skeletal muscle oxidative capacity or reduce ROS production in older adults.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.14814/phy2.12849en
dc.identifier.issue13en
dc.identifier.urihttp://hdl.handle.net/10919/73472en
dc.identifier.volume4en
dc.language.isoenen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/27405968en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectMetabolismen
dc.subjectmitochondriaen
dc.subjectoxidative damageen
dc.subjectstrengthen
dc.subjectAbsorptiometry, Photonen
dc.subjectAge Factorsen
dc.subjectAgeden
dc.subjectAgingen
dc.subjectBiomarkersen
dc.subjectBiopsyen
dc.subjectBody Compositionen
dc.subjectEnergy Metabolismen
dc.subjectGene Expression Regulationen
dc.subjectHumansen
dc.subjectIn Vitro Techniquesen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectMitochondria, Muscleen
dc.subjectMitochondrial Proteinsen
dc.subjectMuscle Contractionen
dc.subjectMuscle Strengthen
dc.subjectOxidation-Reductionen
dc.subjectQuadriceps Muscleen
dc.subjectReactive Oxygen Speciesen
dc.subjectResistance Trainingen
dc.subjectTime Factorsen
dc.titleResistance exercise training and in vitro skeletal muscle oxidative capacity in older adultsen
dc.title.serialPhysiological Reportsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherRandomized Controlled Trialen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Human Nutrition, Foods, & Exerciseen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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