Comparative analysis of Anopheles gambiae L-tyrosine decarboxylase and L-DOPA decarboxylase

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dc.contributor.authorAljabri, Hareb Mohammeden
dc.contributor.committeechairLi, Jianyongen
dc.contributor.committeememberLarson, Timothy J.en
dc.contributor.committeememberTu, Zhijian Jakeen
dc.contributor.departmentBiochemistryen
dc.date.accessioned2014-03-14T20:44:17Zen
dc.date.adate2010-09-14en
dc.date.available2014-03-14T20:44:17Zen
dc.date.issued2010-08-16en
dc.date.rdate2012-04-24en
dc.date.sdate2010-08-26en
dc.description.abstractA major pathway of tyramine and dopamine synthesis in insects is through the decarboxylation of tyrosine and DOPA, respectively. Although tyrosine decarboxylase (TDC) has been mentioned in some reports, it has never been critically analyzed. The high sequence identity shared by tyrosine decarboxylase and DOPA decarboxylase in insects, and the similar structures of the substrates, tyrosine and DOPA, raise the possibility that both tyrosine decarboxylase and DOPA decarboxylase (DDC) have activities to tyrosine and DOPA. In this study, after tyrosine decarboxylase and DOPA decarboxylase enzymes of Anopheles gambiae were expressed, their substrate specificities and biochemical properties were critically analyzed. My results provide clear biochemical evidence establishing that the mosquito tyrosine decarboxylase functions primarily on the production of tyramine with low activity to DOPA. In contrast, mosquito DOPA decarboxylase is highly specific to DOPA with essentially no activity to tyrosine.en
dc.description.degreeMaster of Science in Life Sciencesen
dc.identifier.otheretd-08262010-121034en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-08262010-121034/en
dc.identifier.urihttp://hdl.handle.net/10919/34788en
dc.publisherVirginia Techen
dc.relation.haspartAljabri_HM_T_2010.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectTyrosineen
dc.subjectDOPA decarboxylase (DDC)en
dc.subjectTyrosine decarboxylase (TDC)en
dc.subjectTyramineen
dc.subjectDopamineen
dc.titleComparative analysis of Anopheles gambiae L-tyrosine decarboxylase and L-DOPA decarboxylaseen
dc.typeThesisen
thesis.degree.disciplineBiochemistryen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Science in Life Sciencesen

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