Regeneration and Transformation of Impatiens walleriana Using Cotyledonary Node Culture

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dc.contributor.authorBaxter, Aaron Jacoben
dc.contributor.committeechairVeilleux, Richard E.en
dc.contributor.committeememberDan, Yinghuien
dc.contributor.committeememberScoggins, Holly L.en
dc.contributor.departmentHorticultureen
dc.date.accessioned2014-03-14T20:30:34Zen
dc.date.adate2006-01-16en
dc.date.available2014-03-14T20:30:34Zen
dc.date.issued2005-09-08en
dc.date.rdate2006-01-16en
dc.date.sdate2006-01-13en
dc.description.abstractImpatiens walleriana, commonly grown as a herbaceous annual, is susceptible to Impatiens Necrotic Spot Virus (INSV). A lack of resistant cultivars leaves growers with the sole option of destroying infected plants before INSV spreads throughout their entire crop. Therefore, the introduction of INSV resistant cultivars would have the potential to save Impatiens growers a substantial amount of money. Virus resistance has been successfully conveyed in several crops by insertion of pathogen DNA into the host plant. One method of generating transgenic plants involves the use of Agrobacterium-mediated gene transfer. A commonly used technique involves transformation of explant tissue and subsequent regeneration in vitro under aseptic conditions. However, prior to our research there was no regeneration protocol suitable for Agrobacterium-mediated transformation of Impatiens walleriana available. Herein we report the development of a new method for regeneration of Impatiens walleriana using cotyledonary node culture. Using this technique, four regeneration media amended with 1, 3, 5, or 7µM of thidiazuron were evaluated for their ability to induce de novo shoot production in cotyledonary node explants, and evaluated for number of shoots produced per explant. Results showed a significantly greater frequency of regeneration and number of shoots per explant using media amended with 1µM of thidiazuron. This technique has shown to be repeatable and is not susceptible to ploidy instability. Unfortunately, damage to the cotyledonary node explants during Agrobacterium inoculation and transfection prevented regeneration of transformed shoots in several attempts. However, transient GFP expression after transfection of shoot pads derived from cotyledonary nodes with Agrobacterium strain LBA 4404 containing plasmid pHB2829 with nptII and S-GFP was obtained, indicating the possibility for this regeneration protocol to derive stably transformed Impatiens with INSV resistance.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-01132006-151511en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-01132006-151511/en
dc.identifier.urihttp://hdl.handle.net/10919/30931en
dc.publisherVirginia Techen
dc.relation.haspartThesisFinalVersion-1.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectImpatiens wallerianaen
dc.subjectthidiazuronen
dc.subjectgenetic transformationen
dc.subjectcotyledonary nodeen
dc.subjectAgrobacterium tumefaciensen
dc.titleRegeneration and Transformation of Impatiens walleriana Using Cotyledonary Node Cultureen
dc.typeThesisen
thesis.degree.disciplineHorticultureen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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