Restricted Enzooticity of Hepatitis E Virus Genotypes 1 to 4 in the United States

dc.contributor.authorDong, Chenen
dc.contributor.authorMeng, Jihongen
dc.contributor.authorDai, Xingen
dc.contributor.authorLiang, Jiu-Hongen
dc.contributor.authorFeagins, Alicia R.en
dc.contributor.authorMeng, Xiang-Jinen
dc.contributor.authorBelfiore, Natalia M.en
dc.contributor.authorBradford, Carolen
dc.contributor.authorCorn, Joseph L.en
dc.contributor.authorCray, Carolynen
dc.contributor.authorGlass, Gregory E.en
dc.contributor.authorGordon, Melvin L.en
dc.contributor.authorHesse, Richard A.en
dc.contributor.authorMontgomery, Donald L.en
dc.contributor.authorNicholson, William L.en
dc.contributor.authorPilny, Anthony A.en
dc.contributor.authorRamamoorthy, Sheelaen
dc.contributor.authorShaver, Douglas D.en
dc.contributor.authorDrobeniuc, Janen
dc.contributor.authorPurdy, Michael A.en
dc.contributor.authorFields, Howard A.en
dc.contributor.authorKamili, Saleemen
dc.contributor.authorTeo, Chong-Geeen
dc.contributor.departmentVirginia-Maryland Regional College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiologyen
dc.description.abstractHepatitis E is recognized as a zoonosis, and swine are known reservoirs, but how broadly enzootic its causative agent, hepatitis E virus (HEV), is remains controversial. To determine the prevalence of HEV infection in animals, a serological assay with capability to detect anti-HEV-antibody across a wide variety of animal species was devised. Recombinant antigens comprising truncated capsid proteins generated from HEV-subgenomic constructs that represent all four viral genotypes were used to capture anti-HEV in the test sample and as an analyte reporter. To facilitate development and validation of the assay, serum samples were assembled from blood donors (n = 372), acute hepatitis E patients (n = 94), five laboratory animals (rhesus monkey, pig, New Zealand rabbit, Wistar rat, and BALB/c mouse) immunized with HEV antigens, and four pigs experimentally infected with HEV. The assay was then applied to 4,936 sera collected from 35 genera of animals that were wild, feral, domesticated, or otherwise held captive in the United States. Test positivity was determined in 457 samples (9.3%). These originated from: bison (3/65, 4.6%), cattle (174/1,156, 15%), dogs (2/212, 0.9%), Norway rats (2/318, 0.6%), farmed swine (267/648, 41.2%), and feral swine (9/306, 2.9%). Only the porcine samples yielded the highest reactivities. HEV RNA was amplified from one farmed pig and two feral pigs and characterized by nucleotide sequencing to belong to genotype 3. HEV infected farmed swine primarily, and the role of other animals as reservoirs of its zoonotic spread appears to be limited.en
dc.description.sponsorshipNational Science Foundationen
dc.identifier.citationDong, C. et al. (2011). Restricted Enzooticity of Hepatitis E Virus Genotypes 1 to 4 in the United States. Journal of Clinical Microbiology, 49(12), 4164-4172. doi:10.1128/jcm.05481-11en
dc.publisherAmerican Society for Microbiologyen
dc.rightsIn Copyrighten
dc.rights.holderAmerican Society for Microbiologyen
dc.titleRestricted Enzooticity of Hepatitis E Virus Genotypes 1 to 4 in the United Statesen
dc.title.serialJournal of Clinical Microbiologyen
dc.typeArticle - Refereeden


Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
J. Clin. Microbiol.-2011-Dong-4164-72.pdf
671.01 KB
Adobe Portable Document Format