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Use of molecular probes to assess geographic distribution of Pfiesteria species

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dc.contributor.authorRublee, Parke A.en
dc.contributor.authorKempton, Jason W.en
dc.contributor.authorSchaefer, Eric F.en
dc.contributor.authorAllen, Irving C.en
dc.contributor.authorHarris, Janeraen
dc.contributor.authorOldach, David W.en
dc.contributor.authorBowers, Hollyen
dc.contributor.authorTengs, Torsteinen
dc.contributor.authorBurkholder, JoAnn M.en
dc.contributor.authorGlasgow, H. B.en
dc.date.accessioned2017-01-10T00:47:25Zen
dc.date.available2017-01-10T00:47:25Zen
dc.date.issued2001-10-01en
dc.description.abstractWe have developed multiple polymerase chain reaction (PCR)-based methods for the detection of Pfiesteria sp. in cultures and environmental samples. More than 2,100 water and sediment samples from estuarine sites of the U.S. Atlantic and gulf coasts were assayed for the presence of Pfiesteria piscicida Steidinger & Burkholder and Pfiesteria shumwayae Glasgow & Burkholder by PCR probing of extracted DNA. Positive results were found in about 3% of samples derived from routine monitoring of coastal waters and about 8% of sediments. The geographic range of both species was the same, ranging from New York to Texas. Pfiesteria spp. are likely common and generally benign inhabitants of coastal areas, but their presence maintains a potential for fish and human health problems. Key words: molecular probes, PCR, Pfiesteria, toxic dinoflagellates.en
dc.description.versionPublished versionen
dc.format.extent765 - 767 (3) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.2307/3454924en
dc.identifier.issn0091-6765en
dc.identifier.urihttp://hdl.handle.net/10919/74040en
dc.identifier.volume109en
dc.language.isoenen
dc.publisherUS Department of Health and Human Sciences, Public Health Scienceen
dc.relation.ispartofCDC National Conference on Pfiesteria: From Biology to Public Healthen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000171894300016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectEnvironmental Sciencesen
dc.subjectEnvironmental Healthen
dc.subjectToxicologyen
dc.subjectEnvironmental Sciencesen
dc.subjectEcologyen
dc.subjectOccupational Healthen
dc.subjectmolecular probesen
dc.subjectPCRen
dc.subjectPfiesteriaen
dc.subjecttoxic dinoflagellatesen
dc.subjectPISCICIDAen
dc.subjectDINOFLAGELLATEen
dc.subjectPublic healthen
dc.titleUse of molecular probes to assess geographic distribution of Pfiesteria speciesen
dc.title.serialEnvironmental Health Perspectivesen
dc.typeConference proceedingen
dc.type.dcmitypeTexten
pubs.finish-date2000-10-20en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.start-date2000-10-18en

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