Influence of Fungicide Application on Rhizosphere Microbiota Structure and Microbial Secreted Enzymes in Diverse Cannabinoid-Rich Hemp Cultivars

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dc.contributor.authorXu, Junhuanen
dc.contributor.authorKnight, Tysonen
dc.contributor.authorBoone, Donchelen
dc.contributor.authorSaleem, Muhammaden
dc.contributor.authorFinley, Sheree J.en
dc.contributor.authorGauthier, Nicoleen
dc.contributor.authorAyariga, Joseph A.en
dc.contributor.authorAkinrinlola, Rufusen
dc.contributor.authorPulkoski, Melissaen
dc.contributor.authorBritt, Kadieen
dc.contributor.authorTolosa, Tigisten
dc.contributor.authorRosado-Rivera, Yara I.en
dc.contributor.authorIddrisu, Ibrahimen
dc.contributor.authorThweatt, Ivyen
dc.contributor.authorLi, Tingen
dc.contributor.authorZebelo, Simonen
dc.contributor.authorBurrack, Hannahen
dc.contributor.authorThiessen, Lindseyen
dc.contributor.authorHansen, Zachariahen
dc.contributor.authorBernard, Ernesten
dc.contributor.authorKuhar, Thomasen
dc.contributor.authorSamuel-Foo, Michelleen
dc.contributor.authorAjayi, Olufemi S.en
dc.date.accessioned2024-06-14T13:51:05Zen
dc.date.available2024-06-14T13:51:05Zen
dc.date.issued2024-05-28en
dc.date.updated2024-06-13T14:53:47Zen
dc.description.abstractMicrobes and enzymes play essential roles in soil and plant rhizosphere ecosystem functioning. However, fungicides and plant root secretions may impact the diversity and abundance of microbiota structure and enzymatic activities in the plant rhizosphere. In this study, we analyzed soil samples from the rhizosphere of four cannabinoid-rich hemp (<i>Cannabis sativa</i>) cultivars (Otto II, BaOx, Cherry Citrus, and Wife) subjected to three different treatments (natural infection, fungal inoculation, and fungicide treatment). DNA was extracted from the soil samples, 16S rDNA was sequenced, and data were analyzed for diversity and abundance among different fungicide treatments and hemp cultivars. Fungicide treatment significantly impacted the diversity and abundance of the hemp rhizosphere microbiota structure, and it substantially increased the abundance of the phyla Archaea and Rokubacteria. However, the abundances of the phyla Pseudomonadota and Gemmatimonadetes were substantially decreased in treatments with fungicides compared to those without fungicides in the four hemp cultivars. In addition, the diversity and abundance of the rhizosphere microbiota structure were influenced by hemp cultivars. The influence of Cherry Citrus on the diversity and abundance of the hemp rhizosphere microbiota structure was less compared to the other three hemp cultivars (Otto II, BaOx, and Wife). Moreover, fungicide treatment affected enzymatic activities in the hemp rhizosphere. The application of fungicides significantly decreased enzyme abundance in the rhizosphere of all four hemp cultivars. Enzymes such as dehydrogenase, dioxygenase, hydrolase, transferase, oxidase, carboxylase, and peptidase significantly decreased in all the four hemp rhizosphere treated with fungicides compared to those not treated. These enzymes may be involved in the function of metabolizing organic matter and degrading xenobiotics. The ecological significance of these findings lies in the recognition that fungicides impact enzymes, microbiota structure, and the overall ecosystem within the hemp rhizosphere.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationXu, J.; Knight, T.; Boone, D.; Saleem, M.; Finley, S.J.; Gauthier, N.; Ayariga, J.A.; Akinrinlola, R.; Pulkoski, M.; Britt, K.; Tolosa, T.; Rosado-Rivera, Y.I.; Iddrisu, I.; Thweatt, I.; Li, T.; Zebelo, S.; Burrack, H.; Thiessen, L.; Hansen, Z.; Bernard, E.; Kuhar, T.; Samuel-Foo, M.; Ajayi, O.S. Influence of Fungicide Application on Rhizosphere Microbiota Structure and Microbial Secreted Enzymes in Diverse Cannabinoid-Rich Hemp Cultivars. Int. J. Mol. Sci. 2024, 25, 5892.en
dc.identifier.doihttps://doi.org/10.3390/ijms25115892en
dc.identifier.urihttps://hdl.handle.net/10919/119443en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectCannabis sativaen
dc.subjectcannabinoid (CBD)en
dc.subjectdiversityen
dc.subjectabundanceen
dc.subjecthemp rhizosphereen
dc.subjectfungicideen
dc.subjectenzymesen
dc.titleInfluence of Fungicide Application on Rhizosphere Microbiota Structure and Microbial Secreted Enzymes in Diverse Cannabinoid-Rich Hemp Cultivarsen
dc.title.serialInternational Journal of Molecular Scienceen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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