Analysis of the core bacterial community associated with consumer-ready Eastern oysters (Crassostrea virginica)

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dc.contributor.authorHines, Ian S.en
dc.contributor.authorMarkov Madanick, Justinen
dc.contributor.authorSmith, Stephen A.en
dc.contributor.authorKuhn, David D.en
dc.contributor.authorStevens, Ann M.en
dc.contributor.editorFernández Robledo, José A.en
dc.date.accessioned2025-01-10T14:38:28Zen
dc.date.available2025-01-10T14:38:28Zen
dc.date.issued2023-02-22en
dc.date.issued2023-02-22en
dc.description.abstractShellfish, such as the Eastern oyster (Crassostrea virginica), are an important agricultural commodity. Previous research has demonstrated the importance of the native microbiome of oysters against exogenous challenges by non-native pathogens. However, the taxonomic makeup of the oyster microbiome and the impact of environmental factors on it are understudied. Research was conducted quarterly over a calendar year (February 2020 through February 2021) to analyze the taxonomic diversity of bacteria present within the microbiome of consumer-ready-to-eat live Eastern oysters. It was hypothesized that a core group of bacterial species would be present in the microbiome regardless of external factors such as the water temperature at the time of harvest or post-harvesting processing. At each time point, 18 Chesapeake Bay (eastern United States) watershed aquacultured oysters were acquired from a local grocery store, genomic DNA was extracted from the homogenized whole oyster tissues, and the bacterial 16S rRNA gene hypervariable V4 region was PCR-amplified using barcoded primers prior to sequencing via Illumina MiSeq and bioinformatic analysis of the data. A core group of bacteria were identified to be consistently associated with the Eastern oyster, including members of the phyla Firmicutes and Spirochaetota, represented by the families Mycoplasmataceae and Spirochaetaceae, respectively. The phyla Cyanobacterota and Campliobacterota became more predominant in relation to warmer or colder water column temperature, respectively, at the time of oyster harvest.en
dc.description.versionPublished versionen
dc.format.extent15 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN e0281747 (Article number)en
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0281747en
dc.identifier.eissn1932-6203en
dc.identifier.issn1932-6203en
dc.identifier.issue2en
dc.identifier.orcidStevens, Ann [0000-0002-4942-0124]en
dc.identifier.orcidKuhn, David [0000-0003-3368-0375]en
dc.identifier.orcidSmith, Stephen [0000-0003-4104-1899]en
dc.identifier.otherPONE-D-22-28151 (PII)en
dc.identifier.pmid36812164en
dc.identifier.urihttps://hdl.handle.net/10919/124118en
dc.identifier.volume18en
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/36812164en
dc.relation.urihttps://doi.org/10.1371/journal.pone.0281747en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject.meshAnimalsen
dc.subject.meshHumansen
dc.subject.meshBacteriaen
dc.subject.meshWateren
dc.subject.meshRNA, Ribosomal, 16Sen
dc.subject.meshUnited Statesen
dc.subject.meshCrassostreaen
dc.subject.meshMicrobiotaen
dc.titleAnalysis of the core bacterial community associated with consumer-ready Eastern oysters (<i>Crassostrea virginica)</i>en
dc.title.serialPLOS ONEen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2023-01-30en
pubs.organisational-groupVirginia Techen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciencesen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/Food Science and Technologyen
pubs.organisational-groupVirginia Tech/Scienceen
pubs.organisational-groupVirginia Tech/Science/Biological Sciencesen
pubs.organisational-groupVirginia Tech/Veterinary Medicineen
pubs.organisational-groupVirginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-groupVirginia Tech/All T&R Facultyen
pubs.organisational-groupVirginia Tech/Science/COS T&R Facultyen
pubs.organisational-groupVirginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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