VTechWorks staff will be away for the Thanksgiving holiday beginning at noon on Wednesday, November 27, through Friday, November 29. We will resume normal operations on Monday, December 2. Thank you for your patience.
 

Evaluation of Pgg-Glucan, a Novel Immunomodulator, in in Vitro and Ex Vivo Models of Equine Endotoxemia

Simple item page
dc.contributor.authorSykes, Benjamin Williamen
dc.contributor.committeechairFurr, Martin O.en
dc.contributor.committeememberDonaldson, Lydia L.en
dc.contributor.committeememberMcKenzie, Harold C. IIIen
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T20:40:09Zen
dc.date.adate2003-09-01en
dc.date.available2014-03-14T20:40:09Zen
dc.date.issued2003-06-16en
dc.date.rdate2009-10-02en
dc.date.sdate2003-06-17en
dc.description.abstractJustification - Endotoxemia is an important contributor to mortality and loss of use in the horse and results in significant losses to the equine industry on an annual basis. Objective - To determine the effect of PGG-Glucan on the cytokine response to endotoxin in the horse. Animals - Part 1; 6 adult horses. Part 2; 12 adult horses. Procedure - Part 1; Whole blood was collected, aliquoted, and incubated in vitro in four groups; saline control, endotoxin (LPS) (100 ng/ml), PGG-Glucan (0.1, 1.0, 10 and 100 μg/ml) and LPS (100 ng/ml) plus PGG-Glucan (0.1, 1.0, 10 and 100 μgg/ml). Supernatants were collected at 0, 6 and 12 hours and assayed for tumor necrosis factor £\ (TNF£\) activity. Part 2; Horses received either PGG-Glucan (1 mg/kg) or an equal volume of isotonic saline (0.9% NaCl) IV over 15 minutes. Twenty four hours later blood was collected and mononuclear cells isolated for cell culture. Cells were treated with LPS (100 ng/ml) and RNA extractions were performed at 0, 6, 12, 24 and 48 hours. Relative mRNA expression of TNFα, interleukin-1β (IL-1β),, interleukin-10 (IL-10) and interferon-γ (IFN-γ) was determined by reverse transcription and real time polymerase chain reaction. Results - Using an in vitro endotoxin challenge method PGG-Glucan altered the production of TNFα in a dose-dependent manner. PGG-Glucan had no effect upon the ex vivo cytokine mRNA expression of TNFα, IL-1β, IL-10 or IFN-γ. Conclusions and Relevance - Although mild changes were observed in TNFα production in vitro, it is not likely that PGG-Glucan will have a significant effect upon clinical endotoxemia.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-06172003-143114en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-06172003-143114/en
dc.identifier.urihttp://hdl.handle.net/10919/33617en
dc.publisherVirginia Techen
dc.relation.haspartThesis2.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectHorsesen
dc.subjectendotoxemiaen
dc.subjectPGG-Glucanen
dc.titleEvaluation of Pgg-Glucan, a Novel Immunomodulator, in in Vitro and Ex Vivo Models of Equine Endotoxemiaen
dc.typeThesisen
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Thesis2.pdf
Size:
619.66 KB
Format:
Adobe Portable Document Format
Download

Collections

Masters Theses