Subacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (Oreochromis niloticus)

dc.contributor.authorHart, Laura J.en
dc.contributor.committeechairHolladay, Steven D.en
dc.contributor.committeememberSmith, Bonnie J.en
dc.contributor.committeememberSmith, Stephen A.en
dc.contributor.committeememberNagarkatti, Mitzien
dc.contributor.departmentVeterinary Medical Sciencesen
dc.date.accessioned2014-03-14T21:45:59Zen
dc.date.adate2008-09-18en
dc.date.available2014-03-14T21:45:59Zen
dc.date.issued1996-07-15en
dc.date.rdate2008-09-18en
dc.date.sdate2008-09-18en
dc.description.abstractAlterations of immune parameters were investigated in fish exposed to non-overtly toxic levels of three different environmentally relevant chemicals, 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8- tetrachlorodibenzo-p -dioxin (TCDD). Each chemical agent was administered to tilapia in separate experiments by intraperitoneal injection for five consecutive days. Following the final dose, total cellularity and histology of the spleen and pronephros were assessed, as were activity of phagocytic celis contained in these hematopoietic organs. <p>Using chemical doses which produced no clinical toxicity, tilapia exposed to each chemical agent displayed a significant reduction in total cell number of both spleen and pronephros, in most cases in a dose-related manner. Consistent with this observation, splenic and pronephric hypocellularity was confirmed upon histological examination of chemical-treated fish. However, neither superoxide radical production or phagocytosis of splenic or pronephric macrophages was inhibited in either DMBA, lindane, or TCDD exposed fish. Results of this study indicate that depressed total cell number in fish hematopoietic organs may be a more sensitive indicator of exposure to these environmental contaminants than is the activity of macrophages contained within these organs.en
dc.description.degreeMaster of Scienceen
dc.format.extentix, 138 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-09182008-063320en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-09182008-063320/en
dc.identifier.urihttp://hdl.handle.net/10919/44809en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1996.H378.pdfen
dc.relation.isformatofOCLC# 35652800en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectflow cytometryen
dc.subjecthistologyen
dc.subjectimmunosuppressionen
dc.subjectfish hematopoietic organsen
dc.subjectmacrophageen
dc.subject.lccLD5655.V855 1996.H378en
dc.titleSubacute immunotoxic effects of the environmental contaminants 7,12-dimethylbenzanthracene (DMBA), hexachlorocyclohexane (lindane), and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on spleen and pronephros cellularity and morphology and functional activity of macrophages contained in these hemotopoietic organs in the cichlid fish tilapia (<i>Oreochromis niloticus</i>)en
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineVeterinary Medical Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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