Untargeted Metabolomics and Antioxidant Capacities of Muscadine Grape Genotypes during Berry Development

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dc.contributor.authorDarwish, Ahmed G.en
dc.contributor.authorDas, Protiva Ranien
dc.contributor.authorIsmail, Ahmeden
dc.contributor.authorGajjar, Pranavkumaren
dc.contributor.authorBalasubramani, Subramani Paranthamanen
dc.contributor.authorSheikh, Mehboob B.en
dc.contributor.authorTsolova, Violetaen
dc.contributor.authorSherif, Sherif M.en
dc.contributor.authorEl-Sharkawy, Islamen
dc.contributor.departmentAlson H. Smith Jr. Agricultural Research and Extension Centeren
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2021-06-10T19:36:18Zen
dc.date.available2021-06-10T19:36:18Zen
dc.date.issued2021-06-04en
dc.date.updated2021-06-10T13:46:20Zen
dc.description.abstractThree muscadine grape genotypes (<i>Muscadinia rotundifolia</i> (Michx.) Small) were evaluated for their metabolite profiling and antioxidant activities at different berry developmental stages. A total of 329 metabolites were identified using UPLC-TOF-MS analysis (Ultimate 3000LC combined with Q Exactive MS and screened with ESI-MS) in muscadine genotypes throughout different developmental stages. Untargeted metabolomics study revealed the dominant chemical groups as amino acids, organic acids, sugars, and phenolics. Principal component analysis indicated that developmental stages rather than genotypes could explain the variations among the metabolic profiles of muscadine berries. For instance, catechin, epicatechin-3-gallate, and gallic acid were more accumulated in ripening seeds (RIP-S). However, tartaric acid and malonic acid were more abundant during the fruit-set (FS) stage, and malic acid was more abundant in the veraison (V) stage. The variable importance in the projection (VIP &gt; 0.5) in partial least-squares–discriminant analysis described 27 biomarker compounds, representing the muscadine berry metabolome profiles. A heatmap of Pearson’s correlation analysis between the 27 biomarker compounds and antioxidant activities was able to identify nine antioxidant determinants; among them, gallic acid, 4-acetamidobutanoic acid, trehalose, catechine, and epicatechin-3-gallate displayed the highest correlations with different types of antioxidant activities. For instance, DPPH and FRAP conferred a similar antioxidant activity pattern and were highly correlated with gallic acid and 4-acetamidobutanoic acid. This comprehensive study of the metabolomics and antioxidant activities of muscadine berries at different developmental stages is of great reference value for the plant, food, pharmaceutical, and nutraceutical sectors.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationDarwish, A.G.; Das, P.R.; Ismail, A.; Gajjar, P.; Balasubramani, S.P.; Sheikh, M.B.; Tsolova, V.; Sherif, S.M.; El-Sharkawy, I. Untargeted Metabolomics and Antioxidant Capacities of Muscadine Grape Genotypes during Berry Development. Antioxidants 2021, 10, 914.en
dc.identifier.doihttps://doi.org/10.3390/antiox10060914en
dc.identifier.urihttp://hdl.handle.net/10919/103765en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectdevelopmental stagesen
dc.subjectmetabolomicsen
dc.subjectmuscadine genotypesen
dc.subjectnutritional biomarkersen
dc.titleUntargeted Metabolomics and Antioxidant Capacities of Muscadine Grape Genotypes during Berry Developmenten
dc.title.serialAntioxidantsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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