Inhibition of Thiobacillus ferrooxidans using antibiotics and antibacterial substances

dc.contributor.authorKavanaugh, Rathi G.en
dc.contributor.departmentEnvironmental Sciences and Engineeringen
dc.date.accessioned2014-03-14T21:49:50Zen
dc.date.adate2013-11-15en
dc.date.available2014-03-14T21:49:50Zen
dc.date.issued1988-09-05en
dc.date.rdate2013-11-15en
dc.date.sdate2013-11-15en
dc.description.abstractLaboratory experiments were carried out to evaluate the effectiveness of antibacterial substances and antibiotics against Thiobacillus ferrooxidans, the organisms responsible for bacterial mediated acidic mine drainage. Twenty two antibiotics (obtained from Lilly and Co.) and two antibacterial substances were added to: bacterial culture ATCC 19859 grown in 9K medium. Appropriate controls were maintained. Inhibition of iron oxidizing bacteria was recorded in terms of changes in Eh of the medium treated with the compound. Seven antibiotics (A38533A:, A38533B, 197506, 13780, 171541, chloramphenicol and cephalexin) and the two antibacterial substances [N-serve(nitrapyrin) and Dicyandiamide] effectively inhibited the oxidation of Fe²⁻ ions in the medium. The kinetics of Fe²⁻ oxidation with the addition of antibiotics and the antibacterial substances was studied. N-serve [2-chloro-6-(trichloromethyl) pyridine], used as a nitrification inhibitor in agriculture, was highly effective at concentrations greater than 0.1 ml/l. Iron oxidation levels were reduced to levels close to that in uninoculated controls (abiotic oxidation). The use of N-serve to inhibit acid mine drainage (AMD) causing bacteria seems to be both economical and environmentally safe.en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 58 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-11152013-040528en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-11152013-040528/en
dc.identifier.urihttp://hdl.handle.net/10919/45760en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1988.K383.pdfen
dc.relation.isformatofOCLC# 19765482en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1988.K383en
dc.subject.lcshAcid mine drainage -- Preventionen
dc.subject.lcshThiobacillus ferrooxidansen
dc.titleInhibition of Thiobacillus ferrooxidans using antibiotics and antibacterial substancesen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineEnvironmental Sciences and Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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