Comparative Metabolomics of Early Development of the Parasitic Plants Phelipanche aegyptiaca and Triphysaria versicolor

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dc.contributor.authorClermont, Kristenen
dc.contributor.authorWang, Yaxinen
dc.contributor.authorLiu, Simingen
dc.contributor.authorYang, Zhenzhenen
dc.contributor.authordePamphilis, Claude W.en
dc.contributor.authorYoder, John I.en
dc.contributor.authorCollakova, Evaen
dc.contributor.authorWestwood, James H.en
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2019-06-14T12:57:18Zen
dc.date.available2019-06-14T12:57:18Zen
dc.date.issued2019-06-13en
dc.date.updated2019-06-14T07:32:42Zen
dc.description.abstractParasitic weeds of the family Orobanchaceae attach to the roots of host plants via haustoria capable of drawing nutrients from host vascular tissue. The connection of the haustorium to the host marks a shift in parasite metabolism from autotrophy to at least partial heterotrophy, depending on the level of parasite dependence. Species within the family Orobanchaceae span the spectrum of host nutrient dependency, yet the diversity of parasitic plant metabolism remains poorly understood, particularly during the key metabolic shift surrounding haustorial attachment. Comparative profiling of major metabolites in the obligate holoparasite <i>Phelipanche aegyptiaca</i> and the facultative hemiparasite <i>Triphysaria versicolor</i> before and after attachment to the hosts revealed several metabolic shifts implicating remodeling of energy and amino acid metabolism. After attachment, both parasites showed metabolite profiles that were different from their respective hosts. In <i>P. aegyptiaca</i>, prominent changes in metabolite profiles were also associated with transitioning between different tissue types before and after attachment, with aspartate levels increasing significantly after the attachment. Based on the results from <sup>15</sup>N labeling experiments, asparagine and/or aspartate-rich proteins were enriched in host-derived nitrogen in <i>T. versicolor</i>. These results point to the importance of aspartate and/or asparagine in the early stages of attachment in these plant parasites and provide a rationale for targeting aspartate-family amino acid biosynthesis for disrupting the growth of parasitic weeds.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationClermont, K.; Wang, Y.; Liu, S.; Yang, Z.; dePamphilis, C.W.; Yoder, J.I.; Collakova, E.; Westwood, J.H. Comparative Metabolomics of Early Development of the Parasitic Plants Phelipanche aegyptiaca and Triphysaria versicolor. Metabolites 2019, 9, 114.en
dc.identifier.doihttps://doi.org/10.3390/metabo9060114en
dc.identifier.urihttp://hdl.handle.net/10919/90174en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectparasitic planten
dc.subjectheterotrophyen
dc.subjectPhelipanche aegyptiacaen
dc.subjectTriphysaria versicoloren
dc.subjectcentral carbon and nitrogen metabolismen
dc.titleComparative Metabolomics of Early Development of the Parasitic Plants Phelipanche aegyptiaca and Triphysaria versicoloren
dc.title.serialMetabolitesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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