Geminivirus C4/AC4 proteins hijack cellular COAT PROTEIN COMPLEX I for chloroplast targeting and viral infections

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dc.contributor.authorZhao, Wenhaoen
dc.contributor.authorJi, Yinghuaen
dc.contributor.authorZhou, Yijunen
dc.contributor.authorWang, Xiaofengen
dc.date.accessioned2025-01-15T17:59:26Zen
dc.date.available2025-01-15T17:59:26Zen
dc.date.issued2024-08-20en
dc.description.abstractGeminiviruses infect numerous crops and cause extensive agricultural losses worldwide. During viral infection, geminiviral C4/AC4 proteins relocate from the plasma membrane to chloroplasts, where they inhibit the production of host defense signaling molecules. However, mechanisms whereby C4/AC4 proteins are transported to chloroplasts are unknown. We report here that tomato (Solanum lycopersicum) COAT PROTEIN COMPLEX I (COPI) components play a critical role in redistributing Tomato yellow leaf curl virus C4 protein to chloroplasts via an interaction between the C4 and β subunit of COPI. Coexpression of both proteins promotes the enrichment of C4 in chloroplasts that is blocked by a COPI inhibitor. Overexpressing or downregulating gene expression of COPI components promotes or inhibits the viral infection, respectively, suggesting a proviral role of COPI components. COPI components play similar roles in C4/AC4 transport and infections of two other geminiviruses: Beet curly top virus and East African cassava mosaic virus. Our results reveal an unconventional role of COPI components in protein trafficking to chloroplasts during geminivirus infection and suggest a broad-spectrum antiviral strategy in controlling geminivirus infections in plants.en
dc.description.versionPublished versionen
dc.format.extentPages 1826-1839en
dc.format.extent14 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1093/plphys/kiae436en
dc.identifier.eissn1532-2548en
dc.identifier.issn0032-0889en
dc.identifier.issue3en
dc.identifier.orcidWang, Xiaofeng [0000-0002-3850-3274]en
dc.identifier.other7736869 (PII)en
dc.identifier.pmid39162474en
dc.identifier.urihttps://hdl.handle.net/10919/124205en
dc.identifier.volume196en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/39162474en
dc.rightsPublic Domain (U.S.)en
dc.rights.urihttp://creativecommons.org/publicdomain/mark/1.0/en
dc.subject.meshChloroplastsen
dc.subject.meshGeminiviridaeen
dc.subject.meshPlant Proteinsen
dc.subject.meshViral Proteinsen
dc.subject.meshCapsid Proteinsen
dc.subject.meshPlant Diseasesen
dc.subject.meshProtein Transporten
dc.subject.meshBegomovirusen
dc.subject.meshSolanum lycopersicumen
dc.titleGeminivirus C4/AC4 proteins hijack cellular COAT PROTEIN COMPLEX I for chloroplast targeting and viral infectionsen
dc.title.serialPlant Physiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2024-07-17en
pubs.organisational-groupVirginia Techen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciencesen
pubs.organisational-groupVirginia Tech/All T&R Facultyen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-groupVirginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen

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