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Antibacterial efficacy of core-shell nanostructures encapsulating gentamicin against an in vivo intracellular Salmonella model

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dc.contributor.authorRanjan, Ashishen
dc.contributor.authorPothayee, Nikornen
dc.contributor.authorSeleem, Mohamed N.en
dc.contributor.authorTyler, Ronald D.en
dc.contributor.authorBrenseke, Bonnieen
dc.contributor.authorSriranganathan, Nammalwaren
dc.contributor.authorRiffle, Judy S.en
dc.contributor.authorKasimanickam, Ramanathan K.en
dc.date.accessioned2020-09-21T16:18:24Zen
dc.date.available2020-09-21T16:18:24Zen
dc.date.issued2009-01-01en
dc.date.updated2020-09-21T16:18:21Zen
dc.description.abstractPluronic based core-shell nanostructures encapsulating gentamicin were designed in this study. Block copolymers of (PAA(+/-)Na-b-(PEO-b-PPO-b-PEO)-b-PAA(+/-)Na) were blended with PAA(-) Na(+) and complexed with the polycationic antibiotic gentamicin to form nanostructures. Synthesized nanostructures had a hydrodynamic diameter of 210 nm, zeta potentials of -0.7 (+/-0.2), and incorporated approximately 20% by weight of gentamicin. Nanostructures upon co-incubation with J774A.1 macrophage cells showed no adverse toxicity in vitro. Nanostructures administered in vivo either at multiple dosage of 5 microg g(-1) or single dosage of 15 microg g(-1) in AJ-646 mice infected with Salmonella resulted in significant reduction of viable bacteria in the liver and spleen. Histopathological evaluation for concentration-dependent toxicity at a dosage of 15 microg g(-1) revealed mineralized deposits in 50% kidney tissues of free gentamicin-treated mice which in contrast was absent in nanostructure-treated mice. Thus, encapsulation of gentamicin in nanostructures may reduce toxicity and improve in vivo bacterial clearance.en
dc.description.versionPublished versionen
dc.format.extentPages 289-297en
dc.format.extent9 page(s)en
dc.format.mediumPrint-Electronicen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.2147/ijn.s7137en
dc.identifier.eissn1178-2013en
dc.identifier.issn1178-2013en
dc.identifier.orcidSriranganathan, Nammalwar [0000-0002-9232-2888]en
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458]en
dc.identifier.pmid20054433 (pubmed)en
dc.identifier.urihttp://hdl.handle.net/10919/100047en
dc.identifier.volume4en
dc.language.isoenen
dc.publisherDove Medical Pressen
dc.rightsCreative Commons Attribution-NonCommercial 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/en
dc.subjectLife Sciences & Biomedicineen
dc.subjectNanoscience & Nanotechnologyen
dc.subjectPharmacology & Pharmacyen
dc.subjectScience & Technology - Other Topicsen
dc.subjectgentamicinen
dc.subjectcore-shell nanostructuresen
dc.subjectSalmonellaen
dc.subjectPLURONIC(R) BLOCK-COPOLYMERSen
dc.subjectINDUCED NEPHROTOXICITYen
dc.subjectDRUG-DELIVERYen
dc.subjectLIPOSOMESen
dc.subjectNANOPARTICLESen
dc.subjectNANOCARRIERSen
dc.subjectRELEASEen
dc.subject0601 Biochemistry and Cell Biologyen
dc.subject1007 Nanotechnologyen
dc.subject1115 Pharmacology and Pharmaceutical Sciencesen
dc.subjectNanoscience & Nanotechnologyen
dc.subject.meshAnimalsen
dc.subject.meshMiceen
dc.subject.meshSalmonellaen
dc.subject.meshSalmonella Infectionsen
dc.subject.meshGentamicinsen
dc.subject.meshCoated Materials, Biocompatibleen
dc.subject.meshDrug Carriersen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshCrystallizationen
dc.subject.meshMaterials Testingen
dc.subject.meshDrug Compoundingen
dc.subject.meshDiffusionen
dc.subject.meshDose-Response Relationship, Drugen
dc.subject.meshParticle Sizeen
dc.subject.meshSurface Propertiesen
dc.subject.meshNanostructuresen
dc.subject.meshNanomedicineen
dc.titleAntibacterial efficacy of core-shell nanostructures encapsulating gentamicin against an in vivo intracellular Salmonella modelen
dc.title.serialInternational Journal of Nanomedicineen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Chemistryen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Large Animal Clinical Sciencesen

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