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Nicotiana species as surrogate host for studying the pathogenicity of Acidovorax citrulli, the causal agent of bacterial fruit blotch of cucurbits

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dc.contributor.authorTraore, Sy M.en
dc.contributor.authorEckshtain-Levi, Noamen
dc.contributor.authorMiao, Jiaminen
dc.contributor.authorSparks, Anita Castroen
dc.contributor.authorWang, Zhiboen
dc.contributor.authorWang, Kunruen
dc.contributor.authorLi, Qien
dc.contributor.authorBurdman, Saulen
dc.contributor.authorWalcott, Ronen
dc.contributor.authorWelbaum, Gregory E.en
dc.contributor.authorZhao, Bingyuen
dc.date.accessioned2020-01-15T21:21:01Zen
dc.date.available2020-01-15T21:21:01Zen
dc.date.issued2019-06-01en
dc.date.updated2020-01-15T21:20:58Zen
dc.description.abstractBacterial fruit blotch (BFB) caused by Acidovorax citrulli is one of the most important bacterial diseases of cucurbits worldwide. However, the mechanisms associated with A. citrulli pathogenicity and genetics of host resistance have not been extensively investigated. We idenitfied Nicotiana benthamiana and Nicotiana tabacum as surrogate hosts for studying A. citrulli pathogenicity and non-host resistance triggered by type III secreted (T3S) effectors. Two A. citrulli strains, M6 and AAC00-1, that represent the two major groups amongst A. citrulli populations, induced disease symptoms on N. benthamiana, but triggered a hypersensitive response (HR) on N. tabacum plants. Transient expression of 19 T3S effectors from A. citrulli in N. benthamiana leaves revealed that three effectors, Aave_1548, Aave_2708, and Aave_2166, trigger water-soaking-like cell death in N. benthamiana. Aave_1548 knockout mutants of M6 and AAC00-1 displayed reduced virulence on N. benthamiana and melon (Cucumis melo L.). Transient expression of Aave_1548 and Aave_2166 effectors triggered a non-host HR in N. tabacum, which was dependent on the functionality of the immune signalling component, NtSGT1. Hence, employing Nicotiana species as surrogate hosts for studying A. citrulli pathogenicity may help characterize the function of A. citrulli T3S effectors and facilitate the development of new strategies for BFB management.en
dc.description.versionPublished versionen
dc.format.extentPages 800-814en
dc.format.extent15 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1111/mpp.12792en
dc.identifier.isbn1780645341en
dc.identifier.isbn9781780645346en
dc.identifier.issue6en
dc.identifier.orcidWelbaum, Gregory [0000-0001-8694-2856]en
dc.identifier.urihttp://hdl.handle.net/10919/96449en
dc.identifier.volume20en
dc.language.isoenen
dc.publisherWileyen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000469265900004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAcidovorax citrullien
dc.subjectbacterial fruit blotchen
dc.subjecteffector-triggered immunityen
dc.subjectnon-host resistanceen
dc.subjecttobaccoen
dc.subjecttype III effectorsen
dc.subjectwatermelonen
dc.title<i>Nicotiana</i> species as surrogate host for studying the pathogenicity of <i>Acidovorax citrulli</i>, the causal agent of bacterial fruit blotch of cucurbitsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scotten
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutesen

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