Adeno-associated virus rep78 expression in Arabidopsis thaliana

Simple item page
dc.contributor.authorSisco, Danielen
dc.contributor.committeechairLederman, Muriel L.en
dc.contributor.committeememberWinkel, Brenda S. J.en
dc.contributor.committeememberRutherford, Charles L.en
dc.contributor.departmentBiologyen
dc.date.accessioned2011-08-06T14:45:39Zen
dc.date.adate2002-09-24en
dc.date.available2011-08-06T14:45:39Zen
dc.date.issued2002-09-06en
dc.date.rdate2003-09-24en
dc.date.sdate2002-09-22en
dc.description.abstractAdeno-associated virus type 2 (AAV-2) integrates preferentially into a defined site on human chromosome 19, and has been developed as a gene therapy vector. We propose to use this unique recombination event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into Arabidopsis thaliana via Agrobacterium-mediated transformation. PCR and sequence analysis confirmed the presence of rep78 in two plant lines. RT-PCR demonstrated rep78 transcription in one plant line, but protein could not be detected in either line.en
dc.description.degreeMaster of Scienceen
dc.format.mediumETDen
dc.identifier.otheretd-09222002-193552en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-09222002-193552en
dc.identifier.urihttp://hdl.handle.net/10919/9706en
dc.publisherVirginia Techen
dc.relation.haspart03tableofcontents.pdfen
dc.relation.haspart07references.pdfen
dc.relation.haspart08resume.pdfen
dc.relation.haspart04intro.pdfen
dc.relation.haspart06resultsanddiscussion.pdfen
dc.relation.haspart05materialsandmethods.pdfen
dc.relation.haspart01title.pdfen
dc.relation.haspart02abstract.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectplant transformationen
dc.subjectAdeno-associated virusen
dc.titleAdeno-associated virus rep78 expression in Arabidopsis thalianaen
dc.typeThesisen
thesis.degree.disciplineBiologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

Files

Original bundle
Now showing 1 - 5 of 7
Thumbnail Image
Name:
01title.pdf
Size:
11.38 KB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
02abstract.pdf
Size:
19.69 KB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
03tableofcontents.pdf
Size:
20.71 KB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
04intro.pdf
Size:
339.37 KB
Format:
Adobe Portable Document Format
Download
Thumbnail Image
Name:
05materialsandmethods.pdf
Size:
326.17 KB
Format:
Adobe Portable Document Format
Download

Collections

Masters Theses