Intracellular Diversity of WNV within Circulating Avian Peripheral Blood Mononuclear Cells Reveals Host-Dependent Patterns of Polyinfection

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dc.contributor.authorTalmi-Frank, Daliten
dc.contributor.authorByas, Alex D.en
dc.contributor.authorMurrieta, Reyesen
dc.contributor.authorWeger-Lucarelli, Jamesen
dc.contributor.authorRückert, Claudiaen
dc.contributor.authorGallichotte, Emily N.en
dc.contributor.authorYoshimoto, Janna A.en
dc.contributor.authorAllen, Chrisen
dc.contributor.authorBosco-Lauth, Angela M.en
dc.contributor.authorGraham, Barbaraen
dc.contributor.authorFelix, Todd A.en
dc.contributor.authorBrault, Aaron C.en
dc.contributor.authorEbel, Gregory D.en
dc.date.accessioned2023-06-27T17:25:42Zen
dc.date.available2023-06-27T17:25:42Zen
dc.date.issued2023-05-26en
dc.date.updated2023-06-27T13:21:44Zen
dc.description.abstractArthropod-borne virus (arbovirus) populations exist as mutant swarms that are maintained between arthropods and vertebrates. West Nile virus (WNV) population dynamics are host-dependent. In American crows, purifying selection is weak and population diversity is high compared to American robins, which have 100- to 1000-fold lower viremia. WNV passed in robins leads to fitness gains, whereas that passed in crows does not. Therefore, we tested the hypothesis that high crow viremia allows for higher genetic diversity within individual avian peripheral blood mononuclear cells (PBMCs), reasoning that this could have produced the previously observed host-specific differences in genetic diversity and fitness. Specifically, we infected cells and birds with a molecularly barcoded WNV and sequenced viral RNA from single cells to quantify the number of WNV barcodes in each. Our results demonstrate that the richness of WNV populations within crows far exceeds that in robins. Similarly, rare WNV variants were maintained by crows more frequently than by robins. Our results suggest that increased viremia in crows relative to robins leads to the maintenance of defective genomes and less prevalent variants, presumably through complementation. Our findings further suggest that weaker purifying selection in highly susceptible crows is attributable to this higher viremia, polyinfections and complementation.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationTalmi-Frank, D.; Byas, A.D.; Murrieta, R.; Weger-Lucarelli, J.; Rückert, C.; Gallichotte, E.N.; Yoshimoto, J.A.; Allen, C.; Bosco-Lauth, A.M.; Graham, B.; Felix, T.A.; Brault, A.C.; Ebel, G.D. Intracellular Diversity of WNV within Circulating Avian Peripheral Blood Mononuclear Cells Reveals Host-Dependent Patterns of Polyinfection. Pathogens 2023, 12, 767.en
dc.identifier.doihttps://doi.org/10.3390/pathogens12060767en
dc.identifier.urihttp://hdl.handle.net/10919/115524en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleIntracellular Diversity of WNV within Circulating Avian Peripheral Blood Mononuclear Cells Reveals Host-Dependent Patterns of Polyinfectionen
dc.title.serialPathogensen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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