Improved plaque assay for human coronaviruses 229E and OC43

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dc.contributor.authorBracci, Nicole R.en
dc.contributor.authorPan, Han-Chien
dc.contributor.authorLehman, Caitlin W.en
dc.contributor.authorKehn-Hall, Kyleneen
dc.contributor.authorLin, Shih-Chaoen
dc.date.accessioned2021-10-04T19:20:34Zen
dc.date.available2021-10-04T19:20:34Zen
dc.date.issued2020-12-21en
dc.date.updated2021-10-04T19:20:24Zen
dc.description.abstractIn light of the COVID-19 pandemic, studies that work to understand SARS-CoV-2 are urgently needed. In turn, the less severe human coronaviruses such as HCoV-229E and OC43 are drawing newfound attention. These less severe coronaviruses can be used as a model to facilitate our understanding of the host immune response to coronavirus infection. SARS-CoV-2 must be handled under biosafety level 3 (BSL-3) conditions. Therefore, HCoV-229E and OC43, which can be handled at BSL-2 provide an alternative to SARS-CoV-2 for preclinical screening and designing of antivirals. However, to date, there is no published effective and efficient method to titrate HCoVs other than expensive indirect immunostaining. Here we present an improved approach using an agarose-based conventional plaque assay to titrate HCoV 229E and OC43 with mink lung epithelial cells, Mv1Lu. Our results indicate that titration of HCoV 229E and OC43 with Mv1Lu is consistent and reproducible. The titers produced are also comparable to those produced using human rhabdomyosarcoma (RD) cells. More importantly, Mv1Lu cells display a higher tolerance for cell-cell contact stress, decreased temperature sensitivity, and a faster growth rate. We believe that our improved low-cost plaque assay can serve as an easy tool for researchers conducting HCoV research.en
dc.description.versionPublished versionen
dc.format.extent14 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN e10639 (Article number)en
dc.identifier.doihttps://doi.org/10.7717/peerj.10639en
dc.identifier.eissn2167-8359en
dc.identifier.issn2167-8359en
dc.identifier.orcidKehn-Hall, Kylene [0000-0001-8036-7213]en
dc.identifier.other10639 (PII)en
dc.identifier.pmid33391888en
dc.identifier.urihttp://hdl.handle.net/10919/105160en
dc.identifier.volume8en
dc.language.isoenen
dc.publisherPeerJen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000600832800015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectPlaque assayen
dc.subjectCoronavirusen
dc.subjectAgaroseen
dc.subject229Een
dc.subjectOC43en
dc.subjectSARS-CoV-2en
dc.subjectCOVID-19en
dc.subjectMINK LUNGen
dc.subjectVIRUSen
dc.subjectCELLSen
dc.subjectINFECTIONen
dc.subjectHCOV-229Een
dc.subjectNL63en
dc.subject06 Biological Sciencesen
dc.subject11 Medical and Health Sciencesen
dc.titleImproved plaque assay for human coronaviruses 229E and OC43en
dc.title.serialPeerJen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2020-12-02en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen

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