In vivo metal substitution in bacteroides superoxide dismutase

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dc.contributor.authorChen, Yingen
dc.contributor.committeechairGregory, Eugene M.en
dc.contributor.committeememberBevan, David R.en
dc.contributor.committeememberHess, John L.en
dc.contributor.departmentBiochemistry and Nutritionen
dc.date.accessioned2014-03-14T21:45:07Zen
dc.date.adate2012-09-08en
dc.date.available2014-03-14T21:45:07Zen
dc.date.issued1989-12-08en
dc.date.rdate2012-09-08en
dc.date.sdate2012-09-08en
dc.description.abstractThe effect of various growth conditions on the type of superoxide dismutase (SGD) formed anaerobically in three Bacteriodes species was studied. B. fragilis, B. distasonis, and B. thetaiotaomicron were grown in ironâ restricted media with or without manganese supplementation. Iron availability was decreased by treatment of the media with chelex-100, a metal-chelating resin, and addition of desferrioxamine mesylate (desferal, Ciba-Geigy), an iron chelator. Mn-containing (MnSOD) and Fe-containing superoxide dismutase (EeSOD) activities in cell extracts were differentiated by inhibition with azide and inactivation by H₂0₂. The amount of Mn-containing superoxide dismutase was estimated by the fraction of azide- and H₂0₂, -resistant activity. Cells grown in untreated media contained approximately 90% FeSOD and 10% MnSOD. Cells grown in Fe-restricted media supplemented with graded amounts of manganese synthesized a progressively larger fraction of MnSOD. Hemin, added to the Fe-restricted media, did not serve as an iron source for FeSOD formation. Superoxide dismutase specific activities varied (3-6 U/mg) in each extract but not as a function of manganese concentration.en
dc.description.degreeMaster of Scienceen
dc.format.extentix, 67 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-09082012-040038en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-09082012-040038/en
dc.identifier.urihttp://hdl.handle.net/10919/44628en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1989.C5345.pdfen
dc.relation.isformatofOCLC# 21274277en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1989.C5345en
dc.subject.lcshBacteroidesen
dc.subject.lcshSuperoxide dismutaseen
dc.titleIn vivo metal substitution in bacteroides superoxide dismutaseen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineBiochemistry and Nutritionen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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