Peroxidative protection of parenteral admixture by d-Ã¡-tocopherol and its effect on oxidative status of obese cats
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High lipid : low dextrose (HL:LD) parenteral admixture (PA) is high in polyunsaturated fatty acids (PUFA) that are sensitive to peroxidation. This study evaluated the antioxidative effect of vitamin E in both HL:LD PA and in obese cats given HL:LD PA. Natural d-Ã¡-tocopherol (Vital E-300) was added to HL:LD PA at seven concentrations (8, 12, 16, 24, 32, 48, or 64 IU/g of lipid). PA were exposed to fluorescent light for 24 hours at room temperature. Hydroperoxides were measured at baseline and 24 hours hang time. Significantly lower hydroperoxide concentrations were found with > 24 IU/g of lipid at baseline (P < 0.01). A higher d-Ã¡-tocopherol concentration was required (> 48 IU/g lipid) to lower hydroperoxides at 24 hours (P < 0.0001). HL:LD PA with 40 IU/g lipid/day d-Ã¡-tocopherol was delivered intravenously to obese cats (PA Toc+) over 48 hours. Control cats (PA Toc-) received HL:LD PA without a d-Ã¡-tocopherol supplementation. Oxidative status of cats was evaluated at baseline and 24, 48, and 96 hours. Cats in both groups exhibited an increase in MDA concentration (time effect; P < 0.0001). WBC-tGSH and WBC-GPx did not change in either group of cats. RBC-tGSH and RBC-GPx changed over time (time effects; P = 0.0005; P = 0.0016, respectively) with the PA Toc+ cats exhibiting a higher RBC-tGSH concentration (treatment x time interaction; P = 0.012). Serum Ã¡- and Ã£-tocopherol concentrations increased in PA Toc+ cats (treatment effect; P < 0.0001). These findings suggest that d-Ã¡-tocopherol significantly alters oxidative status in vivo.
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