Peroxidative protection of parenteral admixture by d-α-tocopherol and its effect on oxidative status of obese cats

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dc.contributor.authorBecvarova, Ivetaen
dc.contributor.committeechairSaker, Korinn E.en
dc.contributor.committeememberTroy, Gregory C.en
dc.contributor.committeememberSwecker, William S. Jr.en
dc.contributor.departmentBiomedical and Veterinary Sciencesen
dc.date.accessioned2014-03-14T20:33:25Zen
dc.date.adate2006-06-23en
dc.date.available2014-03-14T20:33:25Zen
dc.date.issued2006-03-30en
dc.date.rdate2010-06-23en
dc.date.sdate2006-04-13en
dc.description.abstractHigh lipid : low dextrose (HL:LD) parenteral admixture (PA) is high in polyunsaturated fatty acids (PUFA) that are sensitive to peroxidation. This study evaluated the antioxidative effect of vitamin E in both HL:LD PA and in obese cats given HL:LD PA. Natural d-α-tocopherol (Vital E-300) was added to HL:LD PA at seven concentrations (8, 12, 16, 24, 32, 48, or 64 IU/g of lipid). PA were exposed to fluorescent light for 24 hours at room temperature. Hydroperoxides were measured at baseline and 24 hours hang time. Significantly lower hydroperoxide concentrations were found with > 24 IU/g of lipid at baseline (P < 0.01). A higher d-α-tocopherol concentration was required (> 48 IU/g lipid) to lower hydroperoxides at 24 hours (P < 0.0001). HL:LD PA with 40 IU/g lipid/day d-α-tocopherol was delivered intravenously to obese cats (PA Toc⁺) over 48 hours. Control cats (PA Toc⁻) received HL:LD PA without a d-α-tocopherol supplementation. Oxidative status of cats was evaluated at baseline and 24, 48, and 96 hours. Cats in both groups exhibited an increase in MDA concentration (time effect; P < 0.0001). WBC-tGSH and WBC-GPx did not change in either group of cats. RBC-tGSH and RBC-GPx changed over time (time effects; P = 0.0005; P = 0.0016, respectively) with the PA Toc⁺ cats exhibiting a higher RBC-tGSH concentration (treatment x time interaction; P = 0.012). Serum α- and γ-tocopherol concentrations increased in PA Toc⁺ cats (treatment effect; P < 0.0001). These findings suggest that d-α-tocopherol significantly alters oxidative status in vivo.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-04132006-103946en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-04132006-103946/en
dc.identifier.urihttp://hdl.handle.net/10919/31707en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartCopyrightPermissionFigure8.pdfen
dc.relation.haspartBecvarovaRevisedThesisPartB.pdfen
dc.relation.haspartBecvarovaRevisedThesisPartA.pdfen
dc.relation.haspartCopyrightPermissionFigure4.pdfen
dc.relation.haspartCopyrightPermissionFigure3.pdfen
dc.relation.haspartCopyrightPermissionFigure1.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectlipid peroxidationen
dc.subjectvitamin Een
dc.subjectObesityen
dc.subjectfelineen
dc.subjectparenteral nutritionen
dc.titlePeroxidative protection of parenteral admixture by d-α-tocopherol and its effect on oxidative status of obese catsen
dc.typeThesisen
thesis.degree.disciplineBiomedical and Veterinary Sciencesen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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