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Detection and Identification of Acinobacillus pleuropneumoniae serotype 5 by multiplex Polymerase Chain Reaction
Traditional serologic assays of Actinobacillus pleuropeumoniae often have
problems with cross-reactivity. To avoid the complications of antibody-antigen
reactions, a PCR assay was developed to detect Actinobacillus pleuropneumoniae
and identify serotype 5 strains. Primers specific to the conserved capsular export
region of A. pleuropneumoniae amplified a 0.7 kb DNA band in all strains with the
exception of serotype 4. A second set of primers specific to the unique capsular
biosynthesis region of serotype 5 amplified a unique 1.1 kb band for serotype 5
only. The sensitivity of this assay was determined to be less than 100 colony
forming units. This PCR assay enables us to detect A. pleuronpeumoniae and definitively
distinguishes serotype 5 strains from other serotyes.