Show simple item record

dc.contributor.authorEleswarapu, Satyanarayana Venkataen_US
dc.date.accessioned2014-03-14T21:36:38Z
dc.date.available2014-03-14T21:36:38Z
dc.date.issued2004-05-11en_US
dc.identifier.otheretd-05272004-100413en_US
dc.identifier.urihttp://hdl.handle.net/10919/42801
dc.description.abstractDevelopmental and Growth Hormone Regulation of the Expression of Liver-Enriched Transcription Factors in the Bovine Liver Satyanarayana Eleswarapu ABSTRACT Liver gene expression changes during development and is affected by growth hormone (GH). These changes in gene expression may be due to the differential expression of the liver-enriched transcription factors (LETFs). To study the potential involvement of LETFs in the regulation of gene expression in the bovine liver, we cloned the cDNA fragments of nine bovine LETFs, including hepatocyte nuclear factor (HNF)-1Æ Ã , 1Æ Ã , 3Æ Ã , 3Æ Ã , 3Æ Ã , 6, albumin D-element binding protein (DBP), and CCAAT/enhancer-binding proteins (C/EBP) -Æ Ã and Æ Ã , and compared the expression levels of them between adult and fetal bovine liver and between GH-treated and untreated adult bovine liver. The mRNA abundance of the LETFs was determined by ribonuclease protection assay (RPA). The cloned bovine LETF cDNA sequences showed high degrees of similarity (79 % to 99 %) to the LETF sequences of other species. The mRNA levels of HNF-1Æ Ã , HNF-3Æ Ã , and HNF-6 were significantly higher (P < 0.05) in the fetal liver (n=3) than in the adult liver (n=7). There were significant increases (P < 0.05) in the mRNA expression of HNF-3Æ Ã and HNF-6 in the liver of cows 24 h (n=6) and 1w (n=6) after GH administration. The results of this study suggest that HNF-1Æ Ã , HNF-3Æ Ã , and HNF-6 may play a role in differential regulation of gene expression between the fetal and adult bovine liver and that HNF-3Æ Ã and HNF-6 may be also involved in GH regulation of gene expression in the bovine liver. Keywords: Liver; Bovine; Growth Hormone; Liver-Enriched Transcription Factorsen_US
dc.publisherVirginia Techen_US
dc.relation.haspartThesisFinal.pdfen_US
dc.rightsI hereby certify that, if appropriate, I have obtained and attached hereto a written permission statement from the owner(s) of each third party copyrighted matter to be included in my thesis, dissertation, or project report, allowing distribution as specified below. I certify that the version I submitted is the same as that approved by my advisory committee. I hereby grant to Virginia Tech or its agents the non-exclusive license to archive and make accessible, under the conditions specified below, my thesis, dissertation, or project report in whole or in part in all forms of media, now or hereafter known. I retain all other ownership rights to the copyright of the thesis, dissertation or project report. I also retain the right to use in future works (such as articles or books) all or part of this thesis, dissertation, or project report.en_US
dc.subjectGrowth Hormoneen_US
dc.subjectBovineen_US
dc.subjectLiveren_US
dc.subjectLiver-Enriched Transcription Factorsen_US
dc.titleDevelopmental and Growth Hormone Regulation of the Expression of Liver-Enriched Transcription Factors in Bovine Liveren_US
dc.typeThesisen_US
dc.contributor.departmentAnimal and Poultry Sciencesen_US
dc.description.degreeMaster of Scienceen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelmastersen_US
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen_US
thesis.degree.disciplineAnimal and Poultry Sciencesen_US
dc.contributor.committeechairJiang, Honglinen_US
dc.contributor.committeememberWong, Eric A.en_US
dc.contributor.committeememberDenbow, Donald Michaelen_US
dc.contributor.committeememberTu, Zhijian Jakeen_US
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05272004-100413/en_US
dc.date.sdate2004-05-27en_US
dc.date.rdate2004-06-22
dc.date.adate2004-06-22en_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record