Aerosol exposure biotesting for package integrity testing
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The objective of this study was to determine how hole diameter, channel length, test organism motility, concentration and aerosol exposure time affected microbiological contamination of sealed flexible pouches. Nickel microtubes with 10 Î¼m and 20Î¼m hole diameters and lengths of 5 mm and 10 mm were used in various combinations to create seal defects in 128 retortable pouches. A 119,911 cm3, exposure chamber was used to distribute an aerosol with a particle size of 2.68 Î¼m, infected with motile and isogenically mutated nonmotile Pseudomonas fragi TM 849 in concentrations of 102 or 106 cells/mL. Fifteen and 30 minute aerosol exposure times were used. Six pouches tested positive for test organism growth after a 72 hour incubation period. Pouch contamination via microbial ingress was significant (P < .05) for test organism motility (motile) and concentration (106 cells/mL).
- Masters Theses