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dc.contributor.authorDeter, Heather S.
dc.contributor.authorJensen, Roderick V.
dc.contributor.authorMather, William H.
dc.contributor.authorButzin, Nicholas C.
dc.date.accessioned2017-09-20T18:33:56Z
dc.date.available2017-09-20T18:33:56Z
dc.date.issued2017-07-04
dc.identifier.citationDeter, H.S.; Jensen, R.V.; Mather, W.H.; Butzin, N.C. Mechanisms for Differential Protein Production in Toxin–Antitoxin Systems. Toxins 2017, 9, 211.
dc.identifier.urihttp://hdl.handle.net/10919/79341
dc.description.abstractToxin–antitoxin (TA) systems are key regulators of bacterial persistence, a multidrug-tolerant state found in bacterial species that is a major contributing factor to the growing human health crisis of antibiotic resistance. Type II TA systems consist of two proteins, a toxin and an antitoxin; the toxin is neutralized when they form a complex. The ratio of antitoxin to toxin is significantly greater than 1.0 in the susceptible population (non-persister state), but this ratio is expected to become smaller during persistence. Analysis of multiple datasets (RNA-seq, ribosome profiling) and results from translation initiation rate calculators reveal multiple mechanisms that ensure a high antitoxin-to-toxin ratio in the non-persister state. The regulation mechanisms include both translational and transcriptional regulation. We classified E. coli type II TA systems into four distinct classes based on the mechanism of differential protein production between toxin and antitoxin. We find that the most common regulation mechanism is translational regulation. This classification scheme further refines our understanding of one of the fundamental mechanisms underlying bacterial persistence, especially regarding maintenance of the antitoxin-to-toxin ratio.
dc.format.mimetypeapplication/pdf
dc.languageen_USen_US
dc.publisherMDPI
dc.rightsCreative Commons Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleMechanisms for Differential Protein Production in Toxin–Antitoxin Systemsen_US
dc.typeArticle - Refereeden_US
dc.date.updated2017-09-20T18:33:56Z
dc.title.serialToxins
dc.identifier.doihttps://doi.org/10.3390/toxins9070211
dc.type.dcmitypeText


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License: Creative Commons Attribution 4.0 International