Thirty-six mature, nongestating, crossbred ewes were randomly allotted to three diets: basal (6 ewes); 50% basal and 50% broiler litter (24 ewes), and 50% basal and 50% broiler litter with the addition of 25 ppm molybdenum (Mo) and 5 g sulfate/kg feed. The 24 litter fed ewes were allotted to withdrawal times of 0-, 30-, 60- and 120-d. The control (basal) diet contained and the deepstacked broiler litter contained 8 and 257 ppm Cu, dry basis, respectively. The ewes were fed the diets for 140-d at which time the control, the six litter-fed ewes with 0-d withdrawal and the Mo and SO₄ supplemented ewes were slaughtered. Liver samples were obtained from these ewes by biopsy initially and at 40-, 80-, and 120-d of the trial. At slaughter liver, kidney, muscle and duodenum samples were taken. Liver Cu content was increased (P<.01) by feeding broiler litter (404 vs 1543 ppm, dry basis). Liver Cu (962 ppm) accumulation and serum Cu were depressed (P<.05) by supplementing litter containing diets with Mo and SO₄. Zinc levels of the liver and Zn and Cu levels of the kidney and muscle were not affected by additional Mo and SO₄. Duodenum Cu and Zn levels reflected the dietary metal content. During the withdrawal period liver and duodenum Cu levels were not lowered (P>.05). A linear increase (P<.001) in SGOT activity was observed with time throughout the withdrawal period, suggesting possible liver damage. Serum Cu decreased at 30-d and increased thereafter (quadratic effect, P<.05). This observation coupled with increases in muscle and kidney Cu (P<.05) indicated an apparent redistribution of Cu stores. Metallothionein (MT) increased 12-fold above control values in the litter-fed ewes (P<.05) and tended to decrease during the withdrawal period. Therefore, it appears that supplementing Mo and SO₄ to a diet containing high Cu broiler litter may reduce the accumulation of hepatic Cu. Liver Cu stores will not be dissipated upon withdrawal of the high Cu litter from the diet.