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dc.contributor.authorVan Eck, L.
dc.contributor.authorDavidson, R. M.
dc.contributor.authorWu, S.
dc.contributor.authorZhao, B. Y.
dc.contributor.authorBotha, A. M.
dc.contributor.authorLeach, J. E.
dc.contributor.authorLapitan, N. L. V.
dc.date.accessioned2020-01-22T04:48:02Z
dc.date.available2020-01-22T04:48:02Z
dc.date.issued2014-01-01
dc.identifier.issn1438-793X
dc.identifier.urihttp://hdl.handle.net/10919/96535
dc.description.abstractThe transcription factor WRKY53 is expressed during biotic and abiotic stress responses in cereals, but little is currently known about its regulation, structure and downstream targets. We sequenced the wheat ortholog TaWRKY53 and its promoter region, which revealed extensive similarity in gene architecture and cis-acting regulatory elements to the rice ortholog OsWRKY53, including the presence of stress-responsive abscisic acid-responsive elements (ABRE) motifs and GCC-boxes. Four proteins interacted with the WRKY53 promoter in yeast one-hybrid assays, suggesting that this gene can receive inputs from diverse stress-related pathways such as calcium signalling and senescence, and environmental cues such as drought and ultraviolet radiation. The Ser/Thr receptor kinase ORK10/LRK10 and the apoplastic peroxidase POC1 are two downstream targets for regulation by the WRKY53 transcription factor, predicted based on the presence of W-box motifs in their promoters and coregulation with WRKY53, and verified by electrophoretic mobility shift assay (EMSA). Both ORK10/LRK10 and POC1 are upregulated during cereal responses to pathogens and aphids and important components of the oxidative burst during the hypersensitive response. Taken with our yeast two-hybrid assay which identified a strong protein-protein interaction between microsomal glutathione S-transferase 3 and WRKY53, this implies that the WRKY53 transcriptional network regulates oxidative responses to a wide array of stresses. © 2014 The Author(s).en
dc.format.extentPages 351-362
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherSpringer
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/24777609
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subject0604 Genetics
dc.subject0607 Plant Biology
dc.subjectPlant Biology & Botany
dc.subject.meshOryza sativa
dc.subject.meshTriticum
dc.subject.meshAbscisic Acid
dc.subject.meshPlant Proteins
dc.subject.meshTranscription Factors
dc.subject.meshUltraviolet Rays
dc.subject.meshSignal Transduction
dc.subject.meshTranscription, Genetic
dc.subject.meshGene Expression Regulation, Plant
dc.subject.meshProtein Binding
dc.subject.meshSequence Homology, Nucleic Acid
dc.subject.meshOxidative Stress
dc.subject.meshGene Regulatory Networks
dc.subject.meshProtein Interaction Domains and Motifs
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshPlant Immunity
dc.subject.meshAbscisic Acid
dc.subject.meshGene Expression Regulation, Plant
dc.subject.meshGene Regulatory Networks
dc.subject.meshOryza
dc.subject.meshOxidative Stress
dc.subject.meshPlant Immunity
dc.subject.meshPlant Proteins
dc.subject.meshPromoter Regions, Genetic
dc.subject.meshProtein Binding
dc.subject.meshProtein Interaction Domains and Motifs
dc.subject.meshSequence Homology, Nucleic Acid
dc.subject.meshSignal Transduction
dc.subject.meshTranscription Factors
dc.subject.meshTranscription, Genetic
dc.subject.meshTriticum
dc.subject.meshUltraviolet Rays
dc.titleThe transcriptional network of WRKY53 in cereals links oxidative responses to biotic and abiotic stress inputsen
dc.typeArticle - Refereed
dc.date.updated2020-01-22T04:47:58Z
dc.description.versionPublished (Publication status)
dc.contributor.departmentSchool of Plant and Environmental Sciencesen_US
dc.title.serialFunctional and Integrative Genomicsen
dc.identifier.doihttps://doi.org/10.1007/s10142-014-0374-3
dc.type.otherJournal Article
dc.identifier.volume14
dc.identifier.issue2
dc.identifier.pmid24777609
dcterms.dateAccepted2014-04-16
dc.identifier.eissn1438-7948
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences
pubs.organisational-group/Virginia Tech/All T&R Faculty
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Faculty
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Durelle Scott
pubs.organisational-group/Virginia Tech
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences
pubs.organisational-group/Virginia Tech/University Research Institutes


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Creative Commons Attribution 4.0 International
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