Neonatal Phosphate Nutrition Alters in Vivo and in Vitro Satellite Cell Activity in Pigs

dc.contributor.authorAlexander, Lindsey S.en
dc.contributor.authorSeabolt, Brynn S.en
dc.contributor.authorRhoads, Robert P.en
dc.contributor.authorStahl, Chad H.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.date.accessioned2017-01-12T20:20:52Zen
dc.date.available2017-01-12T20:20:52Zen
dc.date.issued2012-06-01en
dc.description.abstractSatellite cell activity is necessary for postnatal skeletal muscle growth. Severe phosphate (PO4) deficiency can alter satellite cell activity, however the role of neonatal PO4 nutrition on satellite cell biology remains obscure. Twenty-one piglets (1 day of age, 1.8 ± 0.2 kg BW) were pair-fed liquid diets that were either PO4 adequate (0.9% total P), supra-adequate (1.2% total P) in PO4 requirement or deficient (0.7% total P) in PO4 content for 12 days. Body weight was recorded daily and blood samples collected every 6 days. At day 12, pigs were orally dosed with BrdU and 12 h later, satellite cells were isolated. Satellite cells were also cultured in vitro for 7 days to determine if PO4 nutrition alters their ability to proceed through their myogenic lineage. Dietary PO4 deficiency resulted in reduced (P < 0.05) sera PO4 and parathyroid hormone (PTH) concentrations, while supra-adequate dietary PO4 improved (P < 0.05) feed conversion efficiency as compared to the PO4 adequate group. In vivo satellite cell proliferation was reduced (P < 0.05) among the PO4 deficient pigs, and these cells had altered in vitro expression of markers of myogenic progression. Further work to better understand early nutritional programming of satellite cells and the potential benefits of emphasizing early PO4 nutrition for future lean growth potential is warranted.en
dc.description.versionPublished versionen
dc.format.extent436 - 448 (13) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3390/nu4060436en
dc.identifier.issn2072-6643en
dc.identifier.issue6en
dc.identifier.urihttp://hdl.handle.net/10919/74290en
dc.identifier.volume4en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000305800900002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectNutrition & Dieteticsen
dc.subjectsatellite cellen
dc.subjectphosphateen
dc.subjectneonatalen
dc.subjectpigen
dc.subjectSKELETAL-MUSCLE GROWTHen
dc.subjectPROLIFERATIONen
dc.subjectDIFFERENTIATIONen
dc.subjectDYNAMICSen
dc.subjectNUCLEIen
dc.subjectTURKEYen
dc.subjectRATSen
dc.titleNeonatal Phosphate Nutrition Alters in Vivo and in Vitro Satellite Cell Activity in Pigsen
dc.title.serialNutrientsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Animal and Poultry Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
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