Non-Alcoholic Fatty Liver Disease Induced by Feeding Medium-Chain Fatty Acids Upregulates Cholesterol and Lipid Homeostatic Genes in Skeletal Muscle of Neonatal Pigs

dc.contributor.authorGerrard, Samuel D.en
dc.contributor.authorBiase, Fernando H.en
dc.contributor.authorYonke, Joseph A.en
dc.contributor.authorYadav, Ravien
dc.contributor.authorShafron, Anthony J.en
dc.contributor.authorSunny, Nishanth E.en
dc.contributor.authorGerrard, David E.en
dc.contributor.authorEl-Kadi, Samer W.en
dc.date.accessioned2024-07-29T13:25:24Zen
dc.date.available2024-07-29T13:25:24Zen
dc.date.issued2024-07-11en
dc.date.updated2024-07-26T12:28:58Zen
dc.description.abstractNon-alcoholic fatty liver disease (NAFLD) is a range of disorders characterized by lipid accumulation in hepatocytes. Although this spectrum of disorders is associated with adult obesity, recent evidence suggests that this condition could also occur independently of obesity, even in children. Previously, we reported that pigs fed a formula containing medium-chain fatty acids (MCFAs) developed hepatic steatosis and weighed less than those fed an isocaloric formula containing long-chain fatty acids (LCFAs). Our objective was to determine the association between NAFLD and the skeletal muscle transcriptome in response to energy and lipid intake. Neonatal pigs were fed one of three formulas: a control formula (CONT, <i>n</i> = 6) or one of two isocaloric high-energy formulas containing either long (LCFA, <i>n</i> = 6) or medium (MCFA, <i>n</i> = 6) chain fatty acids. Pigs were fed for 22 d, and tissues were collected. Body weight at 20 and 22 d was greater for LCFA-fed pigs than their CONT or MCFA counterparts (<i>p</i> &lt; 0.005). Longissimus dorsi weight was greater for LCFA compared with MCFA, while CONT was intermediate (<i>p</i> &lt; 0.05). Lean gain and protein deposition were greater for LCFA than for CONT and MCFA groups (<i>p</i> &lt; 0.01). Transcriptomic analysis revealed 36 differentially expressed genes (DEGs) between MCFA and LCFA, 53 DEGs between MCFA and CONT, and 52 DEGs between LCFA and CONT (FDR &lt; 0.2). Feeding formula high in MCFAs resulted in lower body and muscle weights. Transcriptomics data suggest that the reduction in growth was associated with a disruption in cholesterol metabolism in skeletal muscles.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationGerrard, S.D.; Biase, F.H.; Yonke, J.A.; Yadav, R.; Shafron, A.J.; Sunny, N.E.; Gerrard, D.E.; El-Kadi, S.W. Non-Alcoholic Fatty Liver Disease Induced by Feeding Medium-Chain Fatty Acids Upregulates Cholesterol and Lipid Homeostatic Genes in Skeletal Muscle of Neonatal Pigs. Metabolites 2024, 14, 384.en
dc.identifier.doihttps://doi.org/10.3390/metabo14070384en
dc.identifier.urihttps://hdl.handle.net/10919/120728en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectgene expressionen
dc.subjectskeletal muscleen
dc.subjectmetabolismen
dc.subjectNAFLDen
dc.subjectneonatal pigen
dc.titleNon-Alcoholic Fatty Liver Disease Induced by Feeding Medium-Chain Fatty Acids Upregulates Cholesterol and Lipid Homeostatic Genes in Skeletal Muscle of Neonatal Pigsen
dc.title.serialMetabolitesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten

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