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Off-chip passivated-electrode, insulator-based dielectrophoresis (O pi DEP)

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dc.contributor.authorZellner, Phillipen
dc.contributor.authorShake, Tyleren
dc.contributor.authorSahari, Alien
dc.contributor.authorBehkam, Baharehen
dc.contributor.authorAgah, Masouden
dc.contributor.departmentElectrical and Computer Engineeringen
dc.contributor.departmentMechanical Engineeringen
dc.date.accessioned2017-02-12T22:37:42Zen
dc.date.available2017-02-12T22:37:42Zen
dc.date.issued2013-08-01en
dc.description.abstractIn this study, we report the first off-chip passivated-electrode, insulator-based dielectrophoresis microchip (OπDEP). This technique combines the sensitivity of electrode-based dielectrophoresis (eDEP) with the high throughput and inexpensive device characteristics of insulator-based dielectrophoresis (iDEP). The device is composed of a permanent, reusable set of electrodes and a disposable, polymer microfluidic chip with microposts embedded in the microchannel. The device operates by capacitively coupling the electric fields into the microchannel; thus, no physical connections are made between the electrodes and the microfluidic device. During operation, the polydimethylsiloxan (PDMS) microfluidic chip fits onto the electrode substrate as a disposable cartridge. OπDEP uses insulting structures within the channel as well as parallel electrodes to create DEP forces by the same working principle that iDEP devices use. The resulting devices create DEP forces which are larger by two orders of magnitude for the same applied voltage when compared to off-chip eDEP designs from literature, which rely on parallel electrodes alone to produce the DEP forces. The larger DEP forces allow the OπDEP device to operate at high flow rates exceeding 1 mL/h. In order to demonstrate this technology, Escherichia coli (E. coli), a known waterborne pathogen, was trapped from water samples. Trapping efficiencies of 100 % were obtained at flow rates as high as 400 μL/h and 60 % at flow rates as high as 1200 μL/h. Additionally, bacteria were selectively concentrated from a suspension of polystyrene beads.en
dc.description.sponsorshipNational Science Foundationen
dc.description.sponsorshipVirginia Tech Open Access Subvention Funden
dc.description.versionPublisheden
dc.format.extent6657 - 6666 (10) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1007/s00216-013-7123-7en
dc.identifier.issn1618-2642en
dc.identifier.issue21en
dc.identifier.urihttp://hdl.handle.net/10919/75001en
dc.identifier.volume405en
dc.language.isoenen
dc.publisherSpringeren
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000322705600006&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBiochemical Research Methodsen
dc.subjectAnalytical Chemistryen
dc.subjectBiochemistryen
dc.subjectMolecular Biologyen
dc.subjectDielectrophoresis (DEP)en
dc.subjectMicrofluidicen
dc.subjectInsulator-based dielectrophoresis (iDEP)en
dc.subjectEscherichia coli (E. coli)en
dc.subjectMicrofabricationen
dc.titleOff-chip passivated-electrode, insulator-based dielectrophoresis (O pi DEP)en
dc.title.serialAnalytical and Bioanalytical Chemistryen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/COE T&R Facultyen
pubs.organisational-group/Virginia Tech/Engineering/Electrical and Computer Engineeringen
pubs.organisational-group/Virginia Tech/Engineering/Mechanical Engineeringen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen

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