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Early-Life Stress Induced Epigenetic Changes of Corticotropin-Releasing Factor Gene in Anorexic Low Body Weight–Selected Chicks

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dc.contributor.authorXiao, Yangen
dc.contributor.authorWang, Jinxinen
dc.contributor.authorSiegel, Paul B.en
dc.contributor.authorCline, Mark A.en
dc.contributor.authorGilbert, Elizabeth R.en
dc.contributor.departmentAnimal and Poultry Sciencesen
dc.contributor.departmentSchool of Neuroscienceen
dc.date.accessioned2020-04-28T19:00:52Zen
dc.date.available2020-04-28T19:00:52Zen
dc.date.issued2020-04-27en
dc.date.updated2020-04-28T14:14:35Zen
dc.description.abstractThe expression of neuropeptide Y (<i>NPY</i>) in the arcuate nucleus (ARC) and corticotropin-releasing factor (<i>CRF</i>) in the paraventricular nucleus (PVN) were increased when low body weight&ndash;selected (LWS) line chicks, which are predisposed to anorexia, were subjected to a combination of nutritional and thermal stressors at hatch. We hypothesized that such changes resulted from epigenetic modifications. We determined global DNA methylation, DNA methyltransferase (DNMT) activity, and methylation near the promoter regions of <i>NPY</i> and <i>CRF</i>, in the hypothalamus of LWS chicks on day 5 post-hatch. Stress exposure at hatch induced global hypermethylation and increased DNMT activity in the ARC but not PVN. In the PVN of stressed LWS chicks, there was decreased methylation of a CpG site located at the core binding domain of methyl cytosine binding domain protein 2 (MBD2), near the <i>CRF</i> gene promoter. We then demonstrated that this was associated with disrupted binding of MBD2. There was also reduced utilization of yolk reserves and lean and fat masses in chicks that were stress-exposed. These findings provide novel insights on molecular mechanisms through which stressful events induce or intensify anorexia in predisposed individuals and a novel molecular target for further studies.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationXiao, Y.; Wang, J.; Siegel, P.B.; Cline, M.A.; Gilbert, E.R. Early-Life Stress Induced Epigenetic Changes of Corticotropin-Releasing Factor Gene in Anorexic Low Body Weight–Selected Chicks. Life 2020, 10, 51.en
dc.identifier.doihttps://doi.org/10.3390/life10050051en
dc.identifier.urihttp://hdl.handle.net/10919/97931en
dc.language.isoenen
dc.publisherMDPIen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectanorexiaen
dc.subjectARCen
dc.subjectchicksen
dc.subjectCRFen
dc.subjectDNA methylationen
dc.subjectearly-life stressoren
dc.subjecthypothalamusen
dc.subjectMBD2en
dc.subjectNPYen
dc.subjectPVNen
dc.titleEarly-Life Stress Induced Epigenetic Changes of Corticotropin-Releasing Factor Gene in Anorexic Low Body Weight–Selected Chicksen
dc.title.serialLifeen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.dcmitypeStillImageen

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Journal Articles, Multidisciplinary Digital Publishing Institute (MDPI)
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Scholarly Works, School of Animal Sciences
Scholarly Works, School of Neuroscience