Hepatitis E Virus Seroprevalence and Associated Risk Factors in Apparently Healthy Individuals from Osun State, Nigeria
| dc.contributor.author | Osundare, Folakemi Abiodun | en |
| dc.contributor.author | Klink, Patrycja | en |
| dc.contributor.author | Majer, Catharina | en |
| dc.contributor.author | Akanbi, Olusola Aanuoluwapo | en |
| dc.contributor.author | Wang, Bo | en |
| dc.contributor.author | Faber, Mirko | en |
| dc.contributor.author | Harms, Dominik | en |
| dc.contributor.author | Bock, C.-Thomas | en |
| dc.contributor.author | Opaleye, Oladele Oluyinka | en |
| dc.date.accessioned | 2020-05-29T12:00:17Z | en |
| dc.date.available | 2020-05-29T12:00:17Z | en |
| dc.date.issued | 2020-05-20 | en |
| dc.date.updated | 2020-05-28T14:08:15Z | en |
| dc.description.abstract | Hepatitis E virus (HEV) infection is a major public health concern in low-income countries, yet incidence and prevalence estimates are often lacking. Serum (<i>n</i> = 653) and faecal (<i>n</i> = 150) samples were collected from apparently healthy individuals using convenience sampling technique in six communities (Ore, Oke-Osun, Osogbo, Ede, Esa-Odo, and Iperindo) from Osun State, Nigeria. Serum samples were analysed for total anti-HEV IgG/IgM and anti-HEV IgM using commercially available HEV ELISA kits. Total anti-HEV positive serum and all stool samples were analysed for HEV RNA by RT-PCR. Overall, 15.0% (<i>n</i> = 98/653) and 3.8% (<i>n</i> = 25/653) of the serum samples were positive for anti-HEV total and IgM antibodies, respectively. Total anti-HEV and IgM in Ore, Oke-Osun, Osogbo, Ede, Esa-Odo, and Iperindo was 21.0% (<i>n</i> = 13/62) and 3.2% (<i>n</i> = 2/62), 19.4% (<i>n</i> = 20/103) and 6.8% (<i>n</i> = 7/103), 11.4% (<i>n</i> = 12/105) and 2.9% (<i>n</i> = 3/105), 8.0% (<i>n</i> = 16/199) and 1.5% (<i>n</i> = 3/199), 22.0% (<i>n</i> = 22/100) and 10.0% (<i>n</i> = 10/100), and 17.9% (<i>n</i> = 15/84) and 0.0% (<i>n</i> = 0/84), respectively. All samples (stool and serum) were HEV RNA negative. Anti-HEV seroprevalence was associated with rural location, increasing age, alcohol consumption, and rearing of animals. This study demonstrated a high anti-HEV seroprevalence in Osun State, indicating the need to implement surveillance and asses the hepatitis E burden in Nigeria. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Osundare, F.A.; Klink, P.; Majer, C.; Akanbi, O.A.; Wang, B.; Faber, M.; Harms, D.; Bock, C.-T.; Opaleye, O.O. Hepatitis E Virus Seroprevalence and Associated Risk Factors in Apparently Healthy Individuals from Osun State, Nigeria. Pathogens 2020, 9, 392. | en |
| dc.identifier.doi | https://doi.org/10.3390/pathogens9050392 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/98601 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | hepatitis E virus | en |
| dc.subject | seroprevalence | en |
| dc.subject | ELISA | en |
| dc.subject | PCR | en |
| dc.subject | risk factors | en |
| dc.title | Hepatitis E Virus Seroprevalence and Associated Risk Factors in Apparently Healthy Individuals from Osun State, Nigeria | en |
| dc.title.serial | Pathogens | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |
| dc.type.dcmitype | StillImage | en |