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Exogenous Auxin Elicits Changes in the Arabidopsis thaliana Root Proteome in a Time-Dependent Manner

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dc.contributor.authorSlade, William O.en
dc.contributor.authorRay, W. Keithen
dc.contributor.authorHildreth, Sherry B.en
dc.contributor.authorWinkel, Brenda S. J.en
dc.contributor.authorHelm, Richard F.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentBiological Sciencesen
dc.date.accessioned2018-01-08T14:22:34Zen
dc.date.available2018-01-08T14:22:34Zen
dc.date.issued2017-07-10en
dc.description.abstractAuxin is involved in many aspects of root development and physiology, including the formation of lateral roots. Improving our understanding of how the auxin response is mediated at the protein level over time can aid in developing a more complete molecular framework of the process. This study evaluates the effects of exogenous auxin treatment on the Arabidopsis root proteome after exposure of young seedlings to auxin for 8, 12, and 24 h, a timeframe permitting the initiation and full maturation of individual lateral roots. Root protein extracts were processed to peptides, fractionated using off-line strong-cation exchange, and analyzed using ultra-performance liquid chromatography and data independent acquisition-based mass spectrometry. Protein abundances were then tabulated using label-free techniques and evaluated for significant changes. Approximately 2000 proteins were identified during the time course experiment, with the number of differences between the treated and control roots increasing over the 24 h time period, with more proteins found at higher abundance with exposure to auxin than at reduced abundance. Although the proteins identified and changing in levels at each time point represented similar biological processes, each time point represented a distinct snapshot of the response. Auxin coordinately regulates many physiological events in roots and does so by influencing the accumulation and loss of distinct proteins in a time-dependent manner. Data are available via ProteomeXchange with the identifier PXD001400.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3390/proteomes5030016en
dc.identifier.issn2227-7382en
dc.identifier.issue3en
dc.identifier.orcidWinkel, BSJ [0000-0003-3481-024X]en
dc.identifier.orcidHelm, RF [0000-0001-5317-0925]en
dc.identifier.urihttp://hdl.handle.net/10919/81602en
dc.identifier.volume5en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/28698516en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectauxinen
dc.subjectlabel-freeen
dc.subjectrooten
dc.subjecttime courseen
dc.titleExogenous Auxin Elicits Changes in the Arabidopsis thaliana Root Proteome in a Time-Dependent Manneren
dc.title.serialProteomesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dcterms.dateAccepted2017-07-04en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Biological Sciencesen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Facultyen

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