Characterization of Mycobacterium avium cytoplasmic membrane proteins with an emphasis on the major cytoplasmic membrane protein

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dc.contributor.authorCarlisle, Glenn E.en
dc.contributor.committeechairFalkinham, Joseph O. IIIen
dc.contributor.committeememberWilkins, Tracy D.en
dc.contributor.committeememberAnderson, Bruce M.en
dc.contributor.departmentMicrobiology and Immunologyen
dc.date.accessioned2014-03-14T21:36:04Zen
dc.date.adate2010-05-11en
dc.date.available2014-03-14T21:36:04Zen
dc.date.issued1991-05-16en
dc.date.rdate2010-05-11en
dc.date.sdate2010-05-11en
dc.description.abstractProteins of the cytoplasmic membrane of Mycobacterium avium were investigated to identify those which were: (1)intrinsic or extrinsic, (2) attached to the cell wall, (3)surface accessible and (4) excreted. In addition sera containing anti-cytoplasmic membrane proteins were obtained and preliminary purification of the cytoplasmic membrane protein was attempted. The predominating cytoplasmic membrane protein of 31,000 daltons (MCMP) was found to be intrinsic, attached to the cell wall and possibly surface accessible. The MCMP was not excreted, even in media in which the MCMP is not found in the cytoplasmic membrane. Other cytoplasmic membrane proteins were also found to be intrinsic; a few were likely to be extrinsic based upon their separation from the membrane in sucrose gradients. Cytoplasmic membrane proteins of 66, 000, 115, 000 and 129 dalton were surface accessible as judged by I 125-Iodobead labeling. Antisera against the HCMP and other cytoplasmic membrane proteins was obtained and will be useful in further cytoplasmic membrane protein characterization. Acetone precipitation of a cytoplasmic membrane preparation was performed to partially purify the MCMP. The data from this study can be used for the development of serodiagnostic reagents for detecting mycobacterial infection.en
dc.description.degreeMaster of Scienceen
dc.format.extentvii, 57 leavesen
dc.format.mediumBTDen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-05112010-020148en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05112010-020148/en
dc.identifier.urihttp://hdl.handle.net/10919/42610en
dc.language.isoenen
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1991.C376.pdfen
dc.relation.isformatofOCLC# 24424589en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subject.lccLD5655.V855 1991.C376en
dc.subject.lcshMembrane proteins -- Researchen
dc.subject.lcshMycobacterium avium -- Researchen
dc.titleCharacterization of Mycobacterium avium cytoplasmic membrane proteins with an emphasis on the major cytoplasmic membrane proteinen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineMicrobiology and Immunologyen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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