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Analysis of T-DNA alleles of flavonoid biosynthesis genes in Arabidopsis ecotype Columbia

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dc.contributor.authorBowerman, Peter A.en
dc.contributor.authorRamirez, Melissa V.en
dc.contributor.authorPrice, Michelle B.en
dc.contributor.authorHelm, Richard F.en
dc.contributor.authorWinkel, Brenda S. J.en
dc.contributor.departmentBiochemistryen
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2017-01-05T13:31:23Zen
dc.date.available2017-01-05T13:31:23Zen
dc.date.issued2012-09-04en
dc.description.abstractBACKGROUND: The flavonoid pathway is a long-standing and important tool for plant genetics, biochemistry, and molecular biology. Numerous flavonoid mutants have been identified in Arabidopsis over the past several decades in a variety of ecotypes. Here we present an analysis of Arabidopsis lines of ecotype Columbia carrying T-DNA insertions in genes encoding enzymes of the central flavonoid pathway. We also provide a comprehensive summary of various mutant alleles for these structural genes that have been described in the literature to date in a wide variety of ecotypes. FINDINGS: The confirmed knockout lines present easily-scorable phenotypes due to altered pigmentation of the seed coat (or testa). Knockouts for seven alleles for six flavonoid biosynthetic genes were confirmed by PCR and characterized by UPLC for altered flavonol content. CONCLUSION: Seven mutant lines for six genes of the central flavonoid pathway were characterized in ecotype, Columbia. These lines represent a useful resource for integrating biochemical and physiological studies with genomic, transcriptomic, and proteomic data, much of which has been, and continues to be, generated in the Columbia background.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationBMC Research Notes. 2012 Sep 04;5(1):485en
dc.identifier.doihttps://doi.org/10.1186/1756-0500-5-485en
dc.identifier.eissn1756-0500en
dc.identifier.urihttp://hdl.handle.net/10919/73950en
dc.identifier.volume5en
dc.language.isoenen
dc.relation.urihttp://www.ncbi.nlm.nih.gov/pubmed/22947320en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderPeter A Bowerman et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectAcyltransferasesen
dc.subjectAllelesen
dc.subjectArabidopsisen
dc.subjectArabidopsis Proteinsen
dc.subjectBiosynthetic Pathwaysen
dc.subjectChromatography, High Pressure Liquiden
dc.subjectCytochrome P-450 Enzyme Systemen
dc.subjectDNA, Bacterialen
dc.subjectEcotypeen
dc.subjectFlavonoidsen
dc.subjectFlavonolsen
dc.subjectIntramolecular Lyasesen
dc.subjectMixed Function Oxygenasesen
dc.subjectMutagenesis, Insertionalen
dc.subjectNADH, NADPH Oxidoreductasesen
dc.subjectOxygenasesen
dc.subjectPigmentationen
dc.subjectPlants, Genetically Modifieden
dc.subjectSeedsen
dc.titleAnalysis of T-DNA alleles of flavonoid biosynthesis genes in Arabidopsis ecotype Columbiaen
dc.title.serialBMC Research Notesen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherResearch Support, Non-U.S. Gov'ten
dc.type.otherResearch Support, U.S. Gov't, Non-P.H.S.en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Biological Sciencesen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen

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