Accession-Level Differentiation of Urushiol Levels, and Identification of Cardanols in Nascent Emerged Poison Ivy Seedlings
| dc.contributor.author | Lott, Aneirin A. | en |
| dc.contributor.author | Baklajian, Emily R. | en |
| dc.contributor.author | Dickinson, Christopher C. | en |
| dc.contributor.author | Collakova, Eva | en |
| dc.contributor.author | Jelesko, John G. | en |
| dc.contributor.department | School of Plant and Environmental Sciences | en |
| dc.date.accessioned | 2019-11-22T17:47:41Z | en |
| dc.date.available | 2019-11-22T17:47:41Z | en |
| dc.date.issued | 2019-11-20 | en |
| dc.date.updated | 2019-11-22T14:48:05Z | en |
| dc.description.abstract | Poison ivy (<i>Toxicodendron radicans</i> (L.) Kuntze) shows accession-level differentiation in a variety of morphometric traits, suggesting local adaptation. To investigate whether the presumed defense compound urushiol also demonstrates accession-level accumulation differences, in vitro nascent germinated poison ivy seedlings from geographically isolated populations were germinated in vitro and then assayed for known urushiol congener accumulation levels. Significant accession-level differences in the accumulation levels of total C15- and C17-, total C15-, total C17-, specific C15 congeners, and specific C17 congeners of urushiol were identified. In addition, hereto novel C15- and C17-urushiol isomers were identified as well. Cardanols are assumed to be the penultimate metabolites giving rise to urushiols, but this assumption was not previously empirically validated. C15-cardanol congeners and isomers corresponding to expected substrates needed to produce the observed C15-urushiol congeners and isomers were identified in the same poison ivy seedling extracts. Total C15-cardanol and C15-cardanol congeners also showed significant accession-level differences. Based on the observed C15-cardanol congeners in poison ivy, the penultimate step in urushiol biosynthesis was proposed to be a cardanol-specific hydroxylase activity. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Lott, A.A.; Baklajian, E.R.; Dickinson, C.C.; Collakova, E.; Jelesko, J.G. Accession-Level Differentiation of Urushiol Levels, and Identification of Cardanols in Nascent Emerged Poison Ivy Seedlings. Molecules 2019, 24, 4213. | en |
| dc.identifier.doi | https://doi.org/10.3390/molecules24234213 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/95845 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | poison ivy | en |
| dc.subject | Toxicodendron radicans | en |
| dc.subject | urushiol | en |
| dc.subject | cardanol | en |
| dc.subject | and cardanol hydroxylase | en |
| dc.title | Accession-Level Differentiation of Urushiol Levels, and Identification of Cardanols in Nascent Emerged Poison Ivy Seedlings | en |
| dc.title.serial | Molecules | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |