Colonization efficiency of multidrug-resistant Neisseria gonorrhoeae in a female mouse model
dc.contributor.author | Kikiowo, Babatomiwa | en |
dc.contributor.author | Bandara, Aloka B. | en |
dc.contributor.author | Abutaleb, Nader S. | en |
dc.contributor.author | Seleem, Mohamed N. | en |
dc.date.accessioned | 2023-11-30T15:31:31Z | en |
dc.date.available | 2023-11-30T15:31:31Z | en |
dc.date.issued | 2023-10-18 | en |
dc.description.abstract | The rapid occurrence of gonococcal resistance to all classes of antibiotics could lead to untreatable gonorrhea. Thus, development of novel anti-Neisseria gonorrhoeae drugs is urgently needed. Neisseria gonorrhoeae FA1090 is the most used in gonococcal infection mouse models because of its natural resistance to streptomycin. Streptomycin inhibits the urogenital commensal flora that permits gonococcal colonization. However, this strain is drug-susceptible and cannot be used to investigate the efficacy of novel agents against multidrug-resistant N. gonorrhoeae. Hence, to test the in vivo efficacy of new therapeutics against N. gonorrhoeae resistant to the frontline antibiotics, azithromycin, or ceftriaxone, we constructed streptomycin-resistant mutants of N. gonorrhoeae CDC-181 (azithromycin-resistant) and WHO-X (ceftriaxone-resistant). We identified the inoculum size needed to successfully colonize mice. Both mutants, CDC-181-rpsLA128G and WHO-X-rpsLA128G, colonized the genital tract of mice for 14 days with 100% colonization observed for at least 7 days. CDC-181-rpsLA128G demonstrated better colonization of the murine genital tract compared to WHO-X-rpsLA128G. Lower inoculum of WHO-X-rpsLA128G (105 and 106 CFU) colonized mice better than higher inoculum. Overall, our results indicate that CDC-181-rpsLA128G and WHO-X-rpsLA128G can colonize the lower genital tract of mice and are suitable to be used in mouse models to investigate the efficacy of antigonococcal agents. | en |
dc.description.version | Accepted version | en |
dc.format.extent | 7 page(s) | en |
dc.format.mimetype | application/pdf | en |
dc.identifier | ARTN ftad030 (Article number) | en |
dc.identifier.doi | https://doi.org/10.1093/femspd/ftad030 | en |
dc.identifier.eissn | 2049-632X | en |
dc.identifier.issn | 2049-632X | en |
dc.identifier.orcid | Bandara, Aloka [0000-0002-2562-1810] | en |
dc.identifier.orcid | Seleem, Mohamed [0000-0003-0939-0458] | en |
dc.identifier.other | 7321571 (PII) | en |
dc.identifier.pmid | 37852672 | en |
dc.identifier.uri | https://hdl.handle.net/10919/116712 | en |
dc.identifier.volume | 81 | en |
dc.language.iso | en | en |
dc.publisher | Oxford University Press | en |
dc.relation.uri | https://www.ncbi.nlm.nih.gov/pubmed/37852672 | en |
dc.rights | In Copyright | en |
dc.rights.uri | http://rightsstatements.org/vocab/InC/1.0/ | en |
dc.subject | Neisseria gonorrhoeae | en |
dc.subject | rpsL gene | en |
dc.subject | gonococcal mouse model | en |
dc.subject | antibiotic resistance | en |
dc.subject | allelic exchange | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Mice | en |
dc.subject.mesh | Neisseria gonorrhoeae | en |
dc.subject.mesh | Gonorrhea | en |
dc.subject.mesh | Disease Models, Animal | en |
dc.subject.mesh | Ceftriaxone | en |
dc.subject.mesh | Azithromycin | en |
dc.subject.mesh | Streptomycin | en |
dc.subject.mesh | Anti-Bacterial Agents | en |
dc.subject.mesh | Microbial Sensitivity Tests | en |
dc.subject.mesh | Female | en |
dc.title | Colonization efficiency of multidrug-resistant <i>Neisseria gonorrhoeae</i> in a female mouse model | en |
dc.title.serial | Pathogens and Disease | en |
dc.type | Article - Refereed | en |
dc.type.dcmitype | Text | en |
dc.type.other | Article | en |
dc.type.other | Journal | en |
dcterms.dateAccepted | 2023-10-17 | en |
pubs.organisational-group | /Virginia Tech | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences/Biochemistry | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences/Human Nutrition, Foods, & Exercise | en |
pubs.organisational-group | /Virginia Tech/Veterinary Medicine | en |
pubs.organisational-group | /Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiology | en |
pubs.organisational-group | /Virginia Tech/Faculty of Health Sciences | en |
pubs.organisational-group | /Virginia Tech/All T&R Faculty | en |
pubs.organisational-group | /Virginia Tech/Veterinary Medicine/CVM T&R Faculty | en |
pubs.organisational-group | /Virginia Tech/Agriculture & Life Sciences/Human Nutrition, Foods, & Exercise/D. Brown Group | en |
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