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Colonization efficiency of multidrug-resistant Neisseria gonorrhoeae in a female mouse model

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dc.contributor.authorKikiowo, Babatomiwaen
dc.contributor.authorBandara, Aloka B.en
dc.contributor.authorAbutaleb, Nader S.en
dc.contributor.authorSeleem, Mohamed N.en
dc.date.accessioned2023-11-30T15:31:31Zen
dc.date.available2023-11-30T15:31:31Zen
dc.date.issued2023-10-18en
dc.description.abstractThe rapid occurrence of gonococcal resistance to all classes of antibiotics could lead to untreatable gonorrhea. Thus, development of novel anti-Neisseria gonorrhoeae drugs is urgently needed. Neisseria gonorrhoeae FA1090 is the most used in gonococcal infection mouse models because of its natural resistance to streptomycin. Streptomycin inhibits the urogenital commensal flora that permits gonococcal colonization. However, this strain is drug-susceptible and cannot be used to investigate the efficacy of novel agents against multidrug-resistant N. gonorrhoeae. Hence, to test the in vivo efficacy of new therapeutics against N. gonorrhoeae resistant to the frontline antibiotics, azithromycin, or ceftriaxone, we constructed streptomycin-resistant mutants of N. gonorrhoeae CDC-181 (azithromycin-resistant) and WHO-X (ceftriaxone-resistant). We identified the inoculum size needed to successfully colonize mice. Both mutants, CDC-181-rpsLA128G and WHO-X-rpsLA128G, colonized the genital tract of mice for 14 days with 100% colonization observed for at least 7 days. CDC-181-rpsLA128G demonstrated better colonization of the murine genital tract compared to WHO-X-rpsLA128G. Lower inoculum of WHO-X-rpsLA128G (105 and 106 CFU) colonized mice better than higher inoculum. Overall, our results indicate that CDC-181-rpsLA128G and WHO-X-rpsLA128G can colonize the lower genital tract of mice and are suitable to be used in mouse models to investigate the efficacy of antigonococcal agents.en
dc.description.versionAccepted versionen
dc.format.extent7 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN ftad030 (Article number)en
dc.identifier.doihttps://doi.org/10.1093/femspd/ftad030en
dc.identifier.eissn2049-632Xen
dc.identifier.issn2049-632Xen
dc.identifier.orcidBandara, Aloka [0000-0002-2562-1810]en
dc.identifier.orcidSeleem, Mohamed [0000-0003-0939-0458]en
dc.identifier.other7321571 (PII)en
dc.identifier.pmid37852672en
dc.identifier.urihttps://hdl.handle.net/10919/116712en
dc.identifier.volume81en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/37852672en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectNeisseria gonorrhoeaeen
dc.subjectrpsL geneen
dc.subjectgonococcal mouse modelen
dc.subjectantibiotic resistanceen
dc.subjectallelic exchangeen
dc.subject.meshAnimalsen
dc.subject.meshMiceen
dc.subject.meshNeisseria gonorrhoeaeen
dc.subject.meshGonorrheaen
dc.subject.meshDisease Models, Animalen
dc.subject.meshCeftriaxoneen
dc.subject.meshAzithromycinen
dc.subject.meshStreptomycinen
dc.subject.meshAnti-Bacterial Agentsen
dc.subject.meshMicrobial Sensitivity Testsen
dc.subject.meshFemaleen
dc.titleColonization efficiency of multidrug-resistant <i>Neisseria gonorrhoeae</i> in a female mouse modelen
dc.title.serialPathogens and Diseaseen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2023-10-17en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biochemistryen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Human Nutrition, Foods, & Exerciseen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Human Nutrition, Foods, & Exercise/D. Brown Groupen

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