Comparative genomics of Lactobacillaceae from the gut of honey bees, Apis mellifera, from the Eastern United States

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dc.contributor.authorBradford, Emma L.en
dc.contributor.authorWax, Noahen
dc.contributor.authorBueren, Emma K.en
dc.contributor.authorWalke, Jenifer B.en
dc.contributor.authorFell, Richarden
dc.contributor.authorBelden, Lisa K.en
dc.contributor.authorHaak, David C.en
dc.coverage.countryUnited Statesen
dc.date.accessioned2023-01-20T18:21:01Zen
dc.date.available2023-01-20T18:21:01Zen
dc.date.issued2022-12-01en
dc.date.updated2023-01-20T14:46:06Zen
dc.description.abstractLactobacillaceae are an important family of lactic acid bacteria that play key roles in the gut microbiome of many animal species. In the honey bee (Apis mellifera) gut microbiome, many species of Lactobacillaceae are found, and there is functionally important strain-level variation in the bacteria. In this study, we completed whole-genome sequencing of 3 unique Lactobacillaceae isolates collected from hives in Virginia, USA. Using 107 genomes of known bee-associated Lactobacillaceae and Limosilactobacillus reuteri as an outgroup, the phylogenetics of the 3 isolates was assessed, and these isolates were identified as novel strains of Apilactobacillus kunkeei, Lactobacillus kullabergensis, and Bombilactobacillus mellis. Genome rearrangements, conserved orthologous genes (COG) categories and potential prophage regions were identified across the 3 novel strains. The new A. kunkeei strain was enriched in genes related to replication, recombination and repair, the L. kullabergensis strain was enriched for carbohydrate transport, and the B. mellis strain was enriched in transcription or transcriptional regulation and in some genes with unknown functions. Prophage regions were identified in the A. kunkeei and L. kullabergensis isolates. These new bee-associated strains add to our growing knowledge of the honey bee gut microbiome, and to Lactobacillaceae genomics more broadly.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.1093/g3journal/jkac286en
dc.identifier.eissn2160-1836en
dc.identifier.issn2160-1836en
dc.identifier.issue12en
dc.identifier.other6798617 (PII)en
dc.identifier.pmid36331337en
dc.identifier.urihttp://hdl.handle.net/10919/113322en
dc.identifier.volume12en
dc.language.isoenen
dc.publisherOxford University Pressen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/36331337en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectApilactobacillusen
dc.subjectBombilactobacillusen
dc.subjectLactobacillusen
dc.subjectbacteriophageen
dc.subjectgut microbiomeen
dc.subjecthoney bee microbiomeen
dc.subjectprophageen
dc.subjectwhole genomeen
dc.subjectBiotechnologyen
dc.subjectHuman Genomeen
dc.subjectGeneticsen
dc.subject1.1 Normal biological development and functioningen
dc.subject1 Underpinning researchen
dc.subject.meshAnimalsen
dc.subject.meshBeesen
dc.subject.meshBacteriaen
dc.subject.meshLactobacillaceaeen
dc.subject.meshGenomicsen
dc.subject.meshPhylogenyen
dc.subject.meshUnited Statesen
dc.subject.meshGastrointestinal Microbiomeen
dc.titleComparative genomics of Lactobacillaceae from the gut of honey bees, <i>Apis mellifera</i>, from the Eastern United Statesen
dc.title.serialG3: Genes, Genomes, Geneticsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherJournal Articleen
dcterms.dateAccepted2022-10-03en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Plant and Environmental Sciencesen

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