An aeroponic culture system for the study of root herbivory on Arabidopsis thaliana

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dc.contributor.authorVaughan, Martha M.en
dc.contributor.authorTholl, Dorotheaen
dc.contributor.authorTokuhisa, James G.en
dc.contributor.departmentBiological Sciencesen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2017-05-26T19:19:38Zen
dc.date.available2017-05-26T19:19:38Zen
dc.date.issued2011-03-10en
dc.description.abstractBackground Plant defense against herbivory has been studied primarily in aerial tissues. However, complex defense mechanisms have evolved in all parts of the plant to combat herbivore attack and these mechanisms are likely to differ in the aerial and subterranean environment. Research investigating defense responses belowground has been hindered by experimental difficulties associated with the accessibility and quality of root tissue and the lack of bioassays using model plants with altered defense profiles. Results We have developed an aeroponic culture system based on a calcined clay substrate that allows insect herbivores to feed on plant roots while providing easy recovery of the root tissue. The culture method was validated by a root-herbivore system developed for Arabidopsis thaliana and the herbivore Bradysia spp. (fungus gnat). Arabidopsis root mass obtained from aeroponically grown plants was comparable to that from other culture systems, and the plants were morphologically normal. Bradysia larvae caused considerable root damage resulting in reduced root biomass and water absorption. After feeding on the aeroponically grown root tissue, the larvae pupated and emerged as adults. Root damage of mature plants cultivated in aeroponic substrate was compared to that of Arabidopsis seedlings grown in potting mix. Seedlings were notably more susceptible to Bradysia feeding than mature plants and showed decreased overall growth and survival rates. Conclusions A root-herbivore system consisting of Arabidopsis thaliana and larvae of the opportunistic herbivore Bradysia spp. has been established that mimics herbivory in the rhizosphere. Bradysia infestation of Arabidopsis grown in this culture system significantly affects plant performance. The culture method will allow simple profiling and in vivo functional analysis of root defenses such as chemical defense metabolites that are released in response to belowground insect attack.en
dc.description.versionPublished versionen
dc.format.extent? - ? (10) page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifier.citationPlant Methods. 2011 Mar 10;7(1):5en
dc.identifier.doihttps://doi.org/10.1186/1746-4811-7-5en
dc.identifier.issn1746-4811en
dc.identifier.orcidTholl, D [0000-0003-2636-6345]en
dc.identifier.orcidTokuhisa, JG [0000-0002-3834-5149]en
dc.identifier.urihttp://hdl.handle.net/10919/77864en
dc.identifier.volume7en
dc.language.isoenen
dc.publisherBiomed Centralen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000288815900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.holderMartha M Vaughan et al.; licensee BioMed Central Ltd.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectBiochemical Research Methodsen
dc.subjectPlant Sciencesen
dc.subjectBiochemistry & Molecular Biologyen
dc.subjectPLANT SCIENCESen
dc.subjectBELOW-GROUND HERBIVORYen
dc.subjectPLANT DEFENSEen
dc.subjectFUNGUS GNATSen
dc.subjectENTOMOPATHOGENIC NEMATODESen
dc.subjectCOPROPHILA DIPTERAen
dc.subjectINDUCED RESPONSESen
dc.subjectBRADYSIAen
dc.subjectSCIARIDAEen
dc.subjectTOLERANCEen
dc.subjectDIVERSITYen
dc.titleAn aeroponic culture system for the study of root herbivory on Arabidopsis thalianaen
dc.title.serialPlant Methodsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Scienceen
pubs.organisational-group/Virginia Tech/Science/Biological Sciencesen
pubs.organisational-group/Virginia Tech/Science/COS T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Facultyen

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