New Cytogenetic Photomap and Molecular Diagnostics for the Cryptic Species of the Malaria Mosquitoes Anopheles messeae and Anopheles daciae from Eurasia
| dc.contributor.author | Artemov, Gleb N. | en |
| dc.contributor.author | Fedorova, Valentina S. | en |
| dc.contributor.author | Karagodin, Dmitriy A. | en |
| dc.contributor.author | Brusentsov, Ilya I. | en |
| dc.contributor.author | Baricheva, Elina M. | en |
| dc.contributor.author | Sharakhov, Igor V. | en |
| dc.contributor.author | Gordeev, Mikhail I. | en |
| dc.contributor.author | Sharakhova, Maria V. | en |
| dc.contributor.department | Entomology | en |
| dc.date.accessioned | 2021-09-27T12:25:58Z | en |
| dc.date.available | 2021-09-27T12:25:58Z | en |
| dc.date.issued | 2021-09-17 | en |
| dc.date.updated | 2021-09-25T23:33:06Z | en |
| dc.description.abstract | The Eurasian malaria vector <i>Anopheles messeae</i> is a widely spread and genetically diverse species. Five widespread polymorphic chromosomal inversions were found in natural populations of this mosquito. A cryptic species, <i>Anopheles daciae,</i> was differentiated from <i>An. messeae</i> by the presence of several nucleotide substitutions in the Internal Transcribed Spacer 2 (ITS2) region of ribosomal DNA. However, because of the absence of a high-quality reference cytogenetic map, the inversion polymorphisms in <i>An. daciae</i> and <i>An. messeae</i> remain poorly understood. Moreover, a recently determined heterogeneity in ITS2 in <i>An. daciae</i> questioned the accuracy of the previously used Restriction Fragment Length Polymorphism (RFLP) assay for species diagnostics. In this study, a standard-universal cytogenetic map was constructed based on orcein stained images of chromosomes from salivary glands for population studies of the chromosomal inversions that can be used for both <i>An. messeae</i> and <i>An. daciae.</i> In addition, a new ITS2-RFLP approach for species diagnostics was developed. Both methods were applied to characterize inversion polymorphism in populations of <i>An. messeae</i> and <i>An. daciae</i> from a single location in Western Siberia in Russia. The analysis demonstrates that cryptic species are remarkably different in their frequencies of chromosomal inversion variants. Our study supports previous observations that <i>An. messeae</i> has higher inversion polymorphism in all autosomes than the cryptic species <i>An. daciae</i>. | en |
| dc.description.version | Published version | en |
| dc.format.mimetype | application/pdf | en |
| dc.identifier.citation | Artemov, G.N.; Fedorova, V.S.; Karagodin, D.A.; Brusentsov, I.I.; Baricheva, E.M.; Sharakhov, I.V.; Gordeev, M.I.; Sharakhova, M.V. New Cytogenetic Photomap and Molecular Diagnostics for the Cryptic Species of the Malaria Mosquitoes Anopheles messeae and Anopheles daciae from Eurasia. Insects 2021, 12, 835. | en |
| dc.identifier.doi | https://doi.org/10.3390/insects12090835 | en |
| dc.identifier.uri | http://hdl.handle.net/10919/105071 | en |
| dc.language.iso | en | en |
| dc.publisher | MDPI | en |
| dc.rights | Creative Commons Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | en |
| dc.subject | mosquito | en |
| dc.subject | cytogenetic map | en |
| dc.subject | inversion polymorphism | en |
| dc.subject | molecular diagnostic | en |
| dc.title | New Cytogenetic Photomap and Molecular Diagnostics for the Cryptic Species of the Malaria Mosquitoes Anopheles messeae and Anopheles daciae from Eurasia | en |
| dc.title.serial | Insects | en |
| dc.type | Article - Refereed | en |
| dc.type.dcmitype | Text | en |