Modification of the Mycotoxin Deoxynivalenol Using Microorganisms Isolated from Environmental Samples

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dc.contributor.authorWilson, Nina M.en
dc.contributor.authorMcMaster, Nicole J.en
dc.contributor.authorGantulga, Dashen
dc.contributor.authorSoyars, Cara L.en
dc.contributor.authorMcCormick, Susan P.en
dc.contributor.authorKnott, Kenen
dc.contributor.authorSenger, Ryan S.en
dc.contributor.authorSchmale, David G. IIIen
dc.contributor.departmentCivil and Environmental Engineeringen
dc.contributor.departmentSchool of Plant and Environmental Sciencesen
dc.date.accessioned2018-01-15T18:24:38Zen
dc.date.available2018-01-15T18:24:38Zen
dc.date.issued2017-04-01en
dc.description.abstractThe trichothecene mycotoxin deoxynivalenol (DON) is a common contaminant of wheat, barley, and maize. New strategies are needed to reduce or eliminate DON in feed and food products. Microorganisms from plant and soil samples collected in Blacksburg, VA, USA, were screened by incubation in a mineral salt media containing 100 μg/mL DON and analysis by gas chromatography mass spectrometry (GC/MS). Two mixed cultures derived from soil samples consistently decreased DON levels in assays using DON as the sole carbon source. Nuclear magnetic resonance (NMR) analysis indicated that 3-keto-4-deoxynivalenol was the major by-product of DON. Via 16S rRNA sequencing, these mixed cultures, including mostly members of the genera Acinetobacter, Leadbetterella, and Gemmata, were revealed. Incubation of one of these mixed cultures with wheat samples naturally contaminated with 7.1 μg/mL DON indicated nearly complete conversion of DON to the less toxic 3-epimer-DON (3-epi-DON). Our work extends previous studies that have demonstrated the potential for bioprospecting for microorganisms from the environment to remediate or modify mycotoxins for commercial applications, such as the reduction of mycotoxins in fuel ethanol co-products.en
dc.description.versionPublished versionen
dc.format.extent11 pagesen
dc.format.mimetypeapplication/pdfen
dc.identifier.citationWilson, N.M.; McMaster, N.; Gantulga, D.; Soyars, C.; McCormick, S.P.; Knott, K.; Senger, R.S.; Schmale, D.G. Modification of the Mycotoxin Deoxynivalenol Using Microorganisms Isolated from Environmental Samples. Toxins 2017, 9, 141.en
dc.identifier.doihttps://doi.org/10.3390/toxins9040141en
dc.identifier.issn2072-6651en
dc.identifier.issue4en
dc.identifier.orcidSchmale, DG [0000-0002-7003-7429]en
dc.identifier.urihttp://hdl.handle.net/10919/81784en
dc.identifier.volume9en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.urihttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000404136900031&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectFood Toxicologyen
dc.subjectmycotoxinen
dc.subjecttrichotheceneen
dc.subjectdeoxynivalenolen
dc.subjectbioprospectingen
dc.subjectdetoxificationen
dc.subjectFusariumen
dc.subjectTRICHOTHECENE MYCOTOXINSen
dc.subjectMICROBIAL DETOXIFICATIONen
dc.subjectWORLDWIDE CONTAMINATIONen
dc.subjectFUSARIUM MYCOTOXINSen
dc.subjectDEGRADATIONen
dc.subjectGRAINSen
dc.subject3-EPI-DEOXYNIVALENOLen
dc.subjectTRANSFORMATIONen
dc.subjectOCHRATOXINen
dc.subjectTOXICOLOGYen
dc.titleModification of the Mycotoxin Deoxynivalenol Using Microorganisms Isolated from Environmental Samplesen
dc.title.serialToxinsen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Biological Systems Engineeringen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Plant Pathology, Physiology, & Weed Scienceen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/University Research Institutesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciencesen
pubs.organisational-group/Virginia Tech/University Research Institutes/Fralin Life Sciences/Fralin Affiliated Facultyen

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