The Effect of Poly-L-Lysine Concentration, Molecular Weight, and Encapsulation Temperature on Microencapsulated Bovine Spermatozoa

dc.contributor.authorFultz, Stanley Wakefielden
dc.contributor.committeechairSaacke, Richard G.en
dc.contributor.committeememberPearson, Ronald E.en
dc.contributor.committeememberAkers, Robert Michaelen
dc.contributor.committeememberKeenan, Thomas W.en
dc.contributor.committeememberWhite, John M.en
dc.contributor.departmentDairy Scienceen
dc.date.accessioned2014-03-14T20:42:12Zen
dc.date.adate2013-07-29en
dc.date.available2014-03-14T20:42:12Zen
dc.date.issued1984-12-15en
dc.date.rdate2013-07-29en
dc.date.sdate2013-07-29en
dc.description.abstractA series of in vitro studies were conducted to evaluate the effect of poly-l-lysine concentration, molecular weight, and encapsulation temperature on the post encapsulation survivability of spermatozoa. Viability of spermatozoa encapsulated at 2012 C using four poly-l-lysine concentrations (.05%, .15%, .25%, and .35%) did not differ over the 8 h incubation period. However, the viability of each of the four treatments was lower than that of the unencapsulated control (p<.05 and p<.01; percentage motility and percentage intact acrosomes, respectively), indicating spermatozoal damage occurred during the encapsulation process. Capsule wall thickness and integrity for the .15%, .25%, and .35% concentrations were greater (p<.Ol) than that of the .05% capsules.en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 65 leavesen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-07292013-082943en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07292013-082943/en
dc.identifier.urihttp://hdl.handle.net/10919/34249en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1984.F847.pdfen
dc.relation.isformatofOCLC# 12498171en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectspermatoza viabilityen
dc.subject.lccLD5655.V855 1984.F847en
dc.subject.lcshDairy cattle -- Artificial inseminationen
dc.subject.lcshMicroencapsulationen
dc.subject.lcshSpermatozoa -- Experimentsen
dc.titleThe Effect of Poly-L-Lysine Concentration, Molecular Weight, and Encapsulation Temperature on Microencapsulated Bovine Spermatozoaen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineDairy Scienceen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen
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