The Effect of Poly-L-Lysine Concentration, Molecular Weight, and Encapsulation Temperature on Microencapsulated Bovine Spermatozoa

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dc.contributor.authorFultz, Stanley Wakefielden
dc.contributor.committeechairSaacke, Richard G.en
dc.contributor.committeememberPearson, Ronald E.en
dc.contributor.committeememberAkers, Robert Michaelen
dc.contributor.committeememberKeenan, Thomas W.en
dc.contributor.committeememberWhite, John M.en
dc.contributor.departmentDairy Scienceen
dc.date.accessioned2014-03-14T20:42:12Zen
dc.date.adate2013-07-29en
dc.date.available2014-03-14T20:42:12Zen
dc.date.issued1984-12-15en
dc.date.rdate2013-07-29en
dc.date.sdate2013-07-29en
dc.description.abstractA series of in vitro studies were conducted to evaluate the effect of poly-l-lysine concentration, molecular weight, and encapsulation temperature on the post encapsulation survivability of spermatozoa. Viability of spermatozoa encapsulated at 2012 C using four poly-l-lysine concentrations (.05%, .15%, .25%, and .35%) did not differ over the 8 h incubation period. However, the viability of each of the four treatments was lower than that of the unencapsulated control (p<.05 and p<.01; percentage motility and percentage intact acrosomes, respectively), indicating spermatozoal damage occurred during the encapsulation process. Capsule wall thickness and integrity for the .15%, .25%, and .35% concentrations were greater (p<.Ol) than that of the .05% capsules.en
dc.description.degreeMaster of Scienceen
dc.format.extentviii, 65 leavesen
dc.format.mimetypeapplication/pdfen
dc.identifier.otheretd-07292013-082943en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07292013-082943/en
dc.identifier.urihttp://hdl.handle.net/10919/34249en
dc.publisherVirginia Techen
dc.relation.haspartLD5655.V855_1984.F847.pdfen
dc.relation.isformatofOCLC# 12498171en
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectspermatoza viabilityen
dc.subject.lccLD5655.V855 1984.F847en
dc.subject.lcshDairy cattle -- Artificial inseminationen
dc.subject.lcshMicroencapsulationen
dc.subject.lcshSpermatozoa -- Experimentsen
dc.titleThe Effect of Poly-L-Lysine Concentration, Molecular Weight, and Encapsulation Temperature on Microencapsulated Bovine Spermatozoaen
dc.typeThesisen
dc.type.dcmitypeTexten
thesis.degree.disciplineDairy Scienceen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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