VTechWorks staff will be away for the Thanksgiving holiday beginning at noon on Wednesday, November 27, through Friday, November 29. We will resume normal operations on Monday, December 2. Thank you for your patience.
 

Creation of Ovalbumin Based Scaffolds for Bone Tissue Regeneration

Simple item page
dc.contributor.authorFarrar, Gabrielleen
dc.contributor.committeechairMorgan, Abby W.en
dc.contributor.committeememberGatenholm, Paulen
dc.contributor.committeememberBarone, Justin R.en
dc.contributor.committeememberDowling, Norman E.en
dc.contributor.departmentMaterials Science and Engineeringen
dc.date.accessioned2014-03-14T20:35:21Zen
dc.date.adate2009-06-02en
dc.date.available2014-03-14T20:35:21Zen
dc.date.issued2009-04-24en
dc.date.rdate2009-06-02en
dc.date.sdate2009-05-06en
dc.description.abstractBio-based materials are a viable alternative to synthetic materials for tissue engineering. Although many bio-based materials have been used, Ovalbumin (OA) has not yet been researched to create 3D structures that promote cellular responses. Micro-porous scaffolds are a promising construct for bone tissue regeneration; therefore OA crosslinked with three different concentrations (10%, 15% and 20%) of glutaraldehyde (GA) was used in this research. After fabrication, a porous morphology was observed using SEM. Average pore sizes were found to be comparable to scaffolds previously shown to promote cellular response. A TNBS assay determined percent crosslinking in the scaffolds, however there was no significant difference in percent crosslinking despite differing GA concentrations used. Possible explanations include an excess of GA was used. Using DSC, a glass transition temperature (Tg) was found for control indicating the scaffolds are amorphous. Average dry and wet compressive strengths were also found. As expected, differing GA concentrations had no significant effect on Tg and average compressive strengths due to an excess used. Scaffolds were mechanically tested at 37°C with no significant difference found; therefore these scaffolds can be used in the body. It was shown through cell studies that MC3T3-E1 pre-osteoblast cells significantly increased in number on the 10% and 15% scaffolds, therefore cell proliferation occurred. Because of a positive cellular response, 10% GA scaffolds were used for differentiation studies that showed an increase in osteocalcin at 21 days and alkaline phosphatase levels for scaffolds cultured for 14 days. Overall OA scaffolds have shown to be a promising 3D construct for bone tissue regeneration.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-05062009-163857en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-05062009-163857/en
dc.identifier.urihttp://hdl.handle.net/10919/32273en
dc.publisherVirginia Techen
dc.relation.haspartG_FARRAR_THESIS.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjecttissue engineeringen
dc.subjectporous scaffoldsen
dc.subjectbiomaterialsen
dc.subjectovalbuminen
dc.titleCreation of Ovalbumin Based Scaffolds for Bone Tissue Regenerationen
dc.typeThesisen
thesis.degree.disciplineMaterials Science and Engineeringen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
G_FARRAR_THESIS.pdf
Size:
9.77 MB
Format:
Adobe Portable Document Format
Download

Collections

Masters Theses