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Critical Issues in the Processing of cDNA Microarray Images

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dc.contributor.authorJouenne, Vincent Y.en
dc.contributor.committeechairHeath, Lenwood S.en
dc.contributor.committeememberStruble, Craig A.en
dc.contributor.committeememberAlscher, Ruth G.en
dc.contributor.departmentComputer Scienceen
dc.date.accessioned2014-03-14T20:41:17Zen
dc.date.adate2001-07-13en
dc.date.available2014-03-14T20:41:17Zen
dc.date.issued2001-06-28en
dc.date.rdate2002-07-13en
dc.date.sdate2001-07-11en
dc.description.abstractMicroarray technology enables simultaneous gene expression level monitoring for thousands of genes. While this technology has now been recognized as a powerful and cost-effective tool for large-scale analysis, the many systematic sources of experimental variations introduce inherent errors in the extracted data. Data is gathered by processing scanned images of microarray slides. Therefore robust image processing is particularly important and has a large impact on downstream analysis. The processing of the scanned images can be subdivided in three phases: gridding, segmentation and data extraction. To measure the gene expression levels, the processing of cDNA microarray images must overcome a large set of issues in these three phases that motivates this study. This study presents automatic gridding methods and compares their performances. Two segmentation techniques already used, the Seeded Region Growing Algorithm and the Mann-Whitney Test, are examined. We present limitations of these techniques. Finally, we studied the data extraction method used in MicroArray Suite (MS), a microarray analysis software, via synthetic images and explain its intricacies.en
dc.description.degreeMaster of Scienceen
dc.identifier.otheretd-07112001-112357en
dc.identifier.sourceurlhttp://scholar.lib.vt.edu/theses/available/etd-07112001-112357/en
dc.identifier.urihttp://hdl.handle.net/10919/33960en
dc.publisherVirginia Techen
dc.relation.haspartCritical_Issues_in_the_Processing_of_cDNA_Microarray_Images.pdfen
dc.rightsIn Copyrighten
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/en
dc.subjectMann-Whitney Testen
dc.subjectImage Processingen
dc.subjectSegmentationen
dc.subjectGene Expressionen
dc.subjectData Extractionen
dc.subjectSeeded Region Growing Algorithmen
dc.subjectAutomatic Griddingen
dc.titleCritical Issues in the Processing of cDNA Microarray Imagesen
dc.typeThesisen
thesis.degree.disciplineComputer Scienceen
thesis.degree.grantorVirginia Polytechnic Institute and State Universityen
thesis.degree.levelmastersen
thesis.degree.nameMaster of Scienceen

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