Bacterial composition of a competitive exclusion product and its correlation with product efficacy at reducing Salmonella in poultry

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dc.contributor.authorLee, Margie D.en
dc.contributor.authorPedroso, Adriana A.en
dc.contributor.authorMaurer, John J.en
dc.date.accessioned2023-01-20T18:32:12Zen
dc.date.available2023-01-20T18:32:12Zen
dc.date.issued2023-01-09en
dc.date.updated2023-01-20T14:55:46Zen
dc.description.abstractThe mature intestinal microbiome is a formidable barrier to pathogen colonization. Day-old chicks seeded with cecal contents of adult hens are resistant to colonization with <i>Salmonella</i>, the basis of competitive exclusion. Competitive exclusion products can include individual microbes but are commonly undefined intestinal communities taken from adult animals and in commercial production is amplified in fermentator and sold commercially in freeze dried lots. While superior to single and multiple species probiotics, reducing <i>Salmonella</i> colonization by multiple logs, undefined products have limited acceptance because of their uncharacterized status. In this study, the bacterial composition of the master stock, preproduction seed stocks and commercial lots of a poultry competitive exclusion product, was defined by 16S rRNA sequence analysis, targeting the 16S rRNA variable region (V1-V3). The samples contained a diversity of genera (22–52 distinct genera) however, the commercial lots displayed less diversity compared to the seeds and the master stock. Community composition varied between seeds and the master stock and was not a good predictor of potency, in terms of log<sub>10</sub> reduction in <i>Salmonella</i> abundance. While there was significant correlation in composition between seeds and their commercial lots, this too was a not a good predictor of potency. There was linear correlation between unclassified <i>Actinobacteria, Peptococcus,</i> and unclassified Erysipelotrichaceae, and <i>Salmonella</i> abundance (<i>r</i><sup>2</sup> > .75) for commercial seeds. However, upon review of the literature, these three genera were not consistently observed across studies or between trials that examined the correlation between intestinal community composition and <i>Salmonella</i> prevalence or abundance.en
dc.description.versionPublished versionen
dc.format.mimetypeapplication/pdfen
dc.identifier.doihttps://doi.org/10.3389/fphys.2022.1043383en
dc.identifier.eissn1664-042Xen
dc.identifier.issn1664-042Xen
dc.identifier.orcidLee, Margie [0000-0002-0646-4274]en
dc.identifier.orcidMaurer, John [0000-0001-8680-7005]en
dc.identifier.urihttp://hdl.handle.net/10919/113323en
dc.identifier.volume13en
dc.language.isoenen
dc.publisherFrontiersen
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectInfectious Diseasesen
dc.subjectDigestive Diseasesen
dc.titleBacterial composition of a competitive exclusion product and its correlation with product efficacy at reducing <i>Salmonella</i> in poultryen
dc.title.serialFrontiers in Physiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/School of Animal Sciencesen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

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