Expression of anti-chikungunya single-domain antibodies in transgenic Aedes aegypti reduces vector competence for chikungunya virus and Mayaro virus

Simple item page
dc.contributor.authorWebb, Emily M.en
dc.contributor.authorCompton, Austinen
dc.contributor.authorRai, Pallavien
dc.contributor.authorChuong, Christinaen
dc.contributor.authorPaulson, Sally L.en
dc.contributor.authorTu, Zhijianen
dc.contributor.authorWeger-Lucarelli, Jamesen
dc.date.accessioned2024-01-17T14:09:36Zen
dc.date.available2024-01-17T14:09:36Zen
dc.date.issued2023-06-12en
dc.description.abstractChikungunya virus (CHIKV) and Mayaro virus (MAYV) are closely related alphaviruses that cause acute febrile illness accompanied by an incapacitating polyarthralgia that can persist for years following initial infection. In conjunction with sporadic outbreaks throughout the sub-tropical regions of the Americas, increased global travel to CHIKV- and MAYV-endemic areas has resulted in imported cases of MAYV, as well as imported cases and autochthonous transmission of CHIKV, within the United States and Europe. With increasing prevalence of CHIKV worldwide and MAYV throughout the Americas within the last decade, a heavy focus has been placed on control and prevention programs. To date, the most effective means of controlling the spread of these viruses is through mosquito control programs. However, current programs have limitations in their effectiveness; therefore, novel approaches are necessary to control the spread of these crippling pathogens and lessen their disease burden. We have previously identified and characterized an anti-CHIKV single-domain antibody (sdAb) that potently neutralizes several alphaviruses including Ross River virus and Mayaro virus. Given the close antigenic relationship between MAYV and CHIKV, we formulated a single defense strategy to combat both emerging arboviruses: we generated transgenic Aedes aegypti mosquitoes that express two camelid-derived anti-CHIKV sdAbs. Following an infectious bloodmeal, we observed significant reduction in CHIKV and MAYV replication and transmission potential in sdAb-expressing transgenic compared to wild-type mosquitoes; thus, this strategy provides a novel approach to controlling and preventing outbreaks of these pathogens that reduce quality of life throughout the tropical regions of the world.en
dc.description.versionPublished versionen
dc.format.extent11 page(s)en
dc.format.mimetypeapplication/pdfen
dc.identifierARTN 1189176 (Article number)en
dc.identifier.doihttps://doi.org/10.3389/fmicb.2023.1189176en
dc.identifier.eissn1664-302Xen
dc.identifier.issn1664-302Xen
dc.identifier.orcidPaulson, Sally [0000-0003-4206-9279]en
dc.identifier.orcidWeger, James [0000-0002-9483-1694]en
dc.identifier.otherPMC10291133en
dc.identifier.pmid37378291en
dc.identifier.urihttps://hdl.handle.net/10919/117376en
dc.identifier.volume14en
dc.language.isoenen
dc.publisherFrontiersen
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/37378291en
dc.rightsCreative Commons Attribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectalphavirusen
dc.subjectchikungunya virus (CHIKV)en
dc.subjectMayaro virus (MAYV)en
dc.subjectsingle-domain antibodies (sdAb)en
dc.subjectAedes aegyptien
dc.subjecttransgenic mosquitoesen
dc.titleExpression of anti-chikungunya single-domain antibodies in transgenic Aedes aegypti reduces vector competence for chikungunya virus and Mayaro virusen
dc.title.serialFrontiers in Microbiologyen
dc.typeArticle - Refereeden
dc.type.dcmitypeTexten
dc.type.otherArticleen
dc.type.otherJournalen
dcterms.dateAccepted2023-05-16en
pubs.organisational-group/Virginia Techen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciencesen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/Entomologyen
pubs.organisational-group/Virginia Tech/Veterinary Medicineen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/Biomedical Sciences and Pathobiologyen
pubs.organisational-group/Virginia Tech/Faculty of Health Sciencesen
pubs.organisational-group/Virginia Tech/All T&R Facultyen
pubs.organisational-group/Virginia Tech/Veterinary Medicine/CVM T&R Facultyen
pubs.organisational-group/Virginia Tech/Agriculture & Life Sciences/CALS T&R Facultyen

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Expression of anti-chikungunya single-domain antibodies in transgenic iAedes aegyptii reduces vector competence for chikungu.pdf
Size:
2.68 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download
License bundle
Now showing 1 - 1 of 1
Name:
license.txt
Size:
1.5 KB
Format:
Plain Text
Description:
Download

Collections

All Faculty Deposits
Scholarly Works, Biochemistry
Scholarly Works, Biomedical Sciences and Pathobiology
Scholarly Works, Center for Emerging, Zoonotic, and Arthropod-borne Pathogens (CeZAP)
Scholarly Works, Entomology
Scholarly Works, Fralin Life Sciences Institute